Study type: Medical/biological study (experimental study)

Exposure of the SH-SY5Y Human Neuroblastoma Cells to 50-Hz Magnetic Field: Comparison Between Two-Dimensional (2D) and Three-Dimensional (3D) In Vitro Cultures. med./bio.

Published in: Mol Neurobiol 2021; 58 (4): 1634-1649

Aim of study (acc. to author)

The effects of exposure of human neuroblastoma cells to a 50 Hz magnetic field on proliferation and differentiation in 2D and 3D cultures should be investigated.

Background/further details

The conventional 2D monolayer cultures were carried out by plating cells in 12-well plates. 3D cultures were created by using commercial 200-μm-thick polystyrene scaffolds.
Cells were divided into the following groups: 1) exposure to the magnetic field in 2D culture, 2) exposure to the magnetic field in 3D culture. For each exposure group, a separate sham exposure group was used. For proliferation experiments, cells were exposed for 72 hours. For dopaminergic differentiation experiments, cells were treated with retinoic acid for 3 days without MF exposure, followed by treatment wirth phorbol 12-myristate 13-acetate for further 3 days in combination with MF exposure.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 72 hours

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration 72 hours
Exposure setup
Exposure source
Setup exposure system consisted of two couples of square coils (two coils for each sub-system, arranged coaxially in Helmholtz configuration); the coil double wire configuration was used for sham exposure, which allowed to obtain a null magnetic field by using currents flowing in opposite directions; the magnetic field had a high homogeneity (95%) in the exposure volume of coils (20 × 20 × 10 cm3); temperature was kept at 37.0 ± 0.2 °C in both setups
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT effective value measured and calculated - -

Reference articles

  • Benassi B et al. (2016): Extremely low frequency magnetic field (ELF-MF) exposure sensitizes SH-SY5Y cells to the pro-Parkinson's disease toxin MPP+.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Intracellular glutathione levels and SOD1 gene expression were significantly reduced in cells exposed to the magnetic field in 3D-cultures (group 2) compared to the sham exposure, whereas no change was observed in cells grown in 2D cultures (group 1). Moreover, a growth arrest and a significant increase in p21, TH, DAT and GAP43 gene expression was only found in group 2 compared to the sham exposure, indicating a synergy with the differentiating agents to stimulate neuroblastoma cell differentiation into a dopaminergic phenotype.
The authors concluded that exposure of human neuroblastoma cells to a 50 Hz magnetic field might change proliferation and differentiation in 3D cultures but not in 2D cultures.

Study character:

Study funded by

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