To investigate whether exposure to extremely low frequency magnetic fields influences the mitochondrial transmembrane potential in neuroblastoma cells and to study the relationship between conformational changes of biological molecules and cell damage.
|Exposure duration||continuous for 4 hours|
|Chamber||cells were exposed in 25 cm² culture flasks or in 96-well plates|
|Setup||samples were placed between the coils at the centre of a uniform field area; exposure system consisted of a couple of Helmholtz coils, with pole pieces of round polar faces; coil radius was 150 mm; coils were put in incubator ( 5% CO2, 95% humidity, 37.1°C)|
|Sham exposure||A sham exposure was conducted.|
In cell cultures exposed to magnetic flux densities of 0.63 mT or higher, the cell viability was signicantly decreased compared to the control group. Additionally, in cells exposed to a magnetic flux density of 1.41 mT, the transmembrane potential was significantly decreased in comparison to the control.
The exposure of 0.81 mT and higher led to significantly changed absorption spectra of the functional groups. These changes suggest conformational changes in proteins, DNA and lipids.
The authors conclude that exposure to extremely low frequency magnetic fields led to conformational changes of different biological molecules in neuroblastoma cells and that these alterations can be associated with a reduction of the mitochondrial transmembrane potential and the cell viability.