Spinal cord injury was experimentally produced by surgical exposure and subsequent crushing of the spinal cord. Rats were divided into the following groups (n=6 each): 1) exposure of rats with spinal cord injury to the magnetic field, 2) spinal cord injury only (control group), 3) surgical exposure of spinal cord only, no injury (process control). A total of two rats from group 1 and one rat from group 2 died during the exposure procedure, and were therefore not included in the analysis. Exposure to the magnetic field started one day after surgery.
Exposure duration: 2 hours/day for 3 weeks
|Exposure duration||2 hours/day for 3 weeks|
|Chamber||polypropylene cages (50 × 20 × 15 cm3)|
|Setup||exposure system consisted of modified Helmholtz coils with 18 and 8 turns of wire wound on circular frames forming two outer and inner coils, respectively; rats were kept in cages in central area of the coils within a uniform magnetic field|
|magnetic flux density||17.96 µT||-||-||-||-|
Food intake was significantly decreased in the control group (group 2; only spinal cord injury) compared to the exposure group (group 1; spinal cord injury and magnetic field exposure) on day 5. Body weight did not show any significant differences between the groups. A significant improvement in bladder function and motor function was observed in group 1 compared to the control group. Histological analyses showed significantly higher tissue sparing at the injury epicenter and a significant decrease in calcium channel expression in group 1 compared to the control group.
The authors concluded that exposure to a 50 Hz magnetic field might promote sensory-motor recovery via attenuation of secondary damage and calcium-mediated excitotoxicity in rats with a mild spinal cord injury.