Study type: Medical/biological study (experimental study)

Effect of Low Intensity Magnetic Field Stimulation on Calcium-Mediated Cytotoxicity After Mild Spinal Cord Contusion Injury in Rats. med./bio.

Published in: Ann Neurosci 2020; 27 (2): 49-56

Aim of study (acc. to author)

The effects of exposure of rats with a mild spinal cord injury to a 50 Hz magnetic field on general body condition, locomotion, pain, and secondary damages should be investigated.

Background/further details

Spinal cord injury was experimentally produced by surgical exposure and subsequent crushing of the spinal cord. Rats were divided into the following groups (n=6 each): 1) exposure of rats with spinal cord injury to the magnetic field, 2) spinal cord injury only (control group), 3) surgical exposure of spinal cord only, no injury (process control). A total of two rats from group 1 and one rat from group 2 died during the exposure procedure, and were therefore not included in the analysis. Exposure to the magnetic field started one day after surgery.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 2 hours/day for 3 weeks

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration 2 hours/day for 3 weeks
Exposure setup
Exposure source
Chamber polypropylene cages (50 × 20 × 15 cm3)
Setup exposure system consisted of modified Helmholtz coils with 18 and 8 turns of wire wound on circular frames forming two outer and inner coils, respectively; rats were kept in cages in central area of the coils within a uniform magnetic field
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 17.96 µT - - - -

Reference articles

  • Manjhi J et al. (2013): Effect of extremely low frequency magnetic field in prevention of spinal cord injury-induced osteoporosis.
  • Kirschvink JL (1992): Uniform magnetic fields and double-wrapped coil systems: improved techniques for the design of bioelectromagnetic experiments.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • before exposure
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

Food intake was significantly decreased in the control group (group 2; only spinal cord injury) compared to the exposure group (group 1; spinal cord injury and magnetic field exposure) on day 5. Body weight did not show any significant differences between the groups. A significant improvement in bladder function and motor function was observed in group 1 compared to the control group. Histological analyses showed significantly higher tissue sparing at the injury epicenter and a significant decrease in calcium channel expression in group 1 compared to the control group.
The authors concluded that exposure to a 50 Hz magnetic field might promote sensory-motor recovery via attenuation of secondary damage and calcium-mediated excitotoxicity in rats with a mild spinal cord injury.

Study character:

Study funded by

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