For the in vivo tests, rats were divided into 4 groups: 1) spinal cord transection only (n=10), 2) spinal cord transection and implantation of iron oxide nanoparticles at the site of injury (n=7), 3) spinal cord transection and exposure to the magnetic field (n=8) and 4) spinal cord transection and nanoparticles implantation and magnetic field exposure (n=14). Exposure to the magnetic field started 24 hours after spinal cord transection. After 5 weeks, animals were killed and histological examinations were conducted.
Exposure duration: continuous for 2 hours/day for 5 weeks
|Exposure duration||continuous for 2 hours/day for 5 weeks|
|magnetic flux density||17.96 µT||-||-||-||-|
Locomotor, sensory and autonomic functions were significantly enhanced in group 4 (iron oxide nanoparticles and magnetic field exposure) compared to groups 1-3.
Histology demonstrated significant recovery of tissue and a reduction in lesion volume in group 4 compared to the other groups. No collagenous scar was observed in this group and nanoparticles were localized intracellularly in the immediate vicinity of the lesion.
The authors conclude that co-exposure of rats with spinal cord transection to a 50 Hz magnetic field and iron oxide nanoparticles might improve the functional recovery of spinal cord transection, what indicates a possible therapeutic use.