MDCK cells were divided into the following groups: 1) exposure to a 1 mT magnetic field (only used for transepithelial electrical impedance test), 2) exposure to a 5 mT magnetic field. For each exposure group, a separate control group was used.
|Exposure 1: 60 Hz|
|Exposure 2: 60 Hz|
|Chamber||all handling processes were performed in a controlled environment inside a chamber, where temperature, CO2 and humidity were regulated|
|Setup||a uniform magnetic field was produced and a maximum temperature increase of 0.4 °C was measured after 12 h exposure to the 5 mT magnetic field|
The protein expression of CLDN-1 was decreased by 90% in the exposure group (group 2) compared with the control, while ZO-1 protein expression increased by 120%. No significant differences were observed in size and shape of the cell nuclei but a significant decline was observed in the density of cell nuclei in exposed cells compared to the control group. There were no significant differences in cell migration and cell proliferation.
The authors concluded that exposure to a 60 Hz magnetic field might have effects on MDCK epithelial cells.