Study type: Medical/biological study (experimental study)

Alteration of tight and adherens junctions on 50-Hz magnetic field exposure in Madin Darby canine kidney (MDCK) cells. med./bio.

Published in: ScientificWorldJournal 2004; 4: 75-82

Aim of study (acc. to author)

The effects of exposure of canine kidney cells to a 50 Hz magnetic field on the quantity and localization of some junctional proteins of adherens and tight junctions should be investigated.

Background/further details

Adherens and tight junctions are sensitive to heat generated by radio frequency fields. However, non-thermal effects are still unclear, so this study was conducted.
Cells were either not exposed (control group) or exposed for 4 (exposure group 1) or 24 h (exposure group 2).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 4 or 24 h

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 4 or 24 h
Exposure setup
Exposure source
Setup a pair of Helmholtz coils with an inner diameter of 12 cm and 85 turns of 0.3 mm copper wire each was placed inside a CO2 incubator with a constant temperature of 37°C; a vertical magnetic field was produced
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 500 µT effective value measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

The location of all investigated proteins was not changed in both exposure groups compared to control, but the amount of membrane-bound proteins seemed to be higher. The total amount of β-catenin was significantly decreased in both exposure groups compared to the control.
The authors conclude that exposure of canine kidney cells to a 50 Hz magnetic field might affect the local and total amount of some junction proteins in cells.

Study character:

Study funded by

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