Study type: Medical/biological study (experimental study)

Effects of an electric field on sleep quality and life span mediated by ultraviolet (UV)-A/blue light photoreceptor CRYPTOCHROME in Drosophila. med./bio.

Published in: Sci Rep 2021; 11 (1): 20543

Aim of study (acc. to author)

The aim of the study was to elucidate the molecular mechanisms underlying electric field effects in Drosophila melanogaster as a genetic animal model. The hypothesis should be tested that an electric field exposure of 50 Hz can improve sleep quality and extend the life span in wild type flies and that cryptochrome is essential for the electric field receptor system in Drosophila melanogaster.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 12, 24 or 48 h (dependent of the experiment); maybe longer (duration not exactly provided for each experiment)

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration 12, 24 or 48 h (dependent of the experiment); maybe longer (duration not exactly provided for each experiment)
Exposure setup
Exposure source
Setup flies and medium were placed in vials or tubes and placed 3–5 cm apart between the electrodes
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 35 kV/m - - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

Exposure to 50 Hz electric fields during the daytime improved sleep quality in wild type flies, whereas nighttime exposure did not. This effect was not observed in cryb mutant flies.
The average lifespan of exposed wild type flies of the low nutritional and the starvation condition was significantly increased by approx. 18 % and 20 %, respectively, compared with that of sham exposed flies. In contrast, electric field exposure did not alter the lifespan under starvation in any of the mutant flies.
Additionally, the data indicated that electric field exposure significantly increased ATP level and several nucleic acid metabolisms in the flies.
The data provide the first genetic evidence of a cryptochrome-based electric field sensitive system in animals.

Study character:

Study funded by

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