Study type: Medical/biological study (experimental study)

Increase of brain ammonia after microwave irradiation and its mechanism. med./bio.

Published in: In Vivo 1989; 3 (5): 339-343

Aim of study (acc. to editor)

After a lethal microwave exposure (4 kW; 2.45 GHz; 0.4-1.2 s) the concentration of different metabolites in mice brain were determined in contrast of mice killed by neck dislocation without microwave exposure.

Background/further details

In addition two enzymes involved in amino acid metabolism, phosphate-dependent glutaminase and glutamine synthetase, were studied. The metabolites of interest were: ammonia, amino acids, adenine nucleotides, glucose, glycogen, lactate and pyruvate. Quick dispatch by microwave exposure of experimental animals is to be considered as a common method to determine the in vitro concentration of rapidly-metabolized substances.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 2.45 GHz
Modulation type: CW
Exposure duration: 0.4 s - 1.5 s

Exposure 1

Main characteristics
Frequency 2.45 GHz
Type
Exposure duration 0.4 s - 1.5 s
Modulation
Modulation type CW
Exposure setup
Exposure source
  • MI-System Toshiba TMW-6402A+
Parameters
Measurand Value Type Method Mass Remarks
power 4 kW - - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The ammonia concentration was found about 5 times higher in animals exposed to 0.4 s than in non or longer exposed animals. Insofar, 4 kW 2.45 GHz exposure of 4 s was determined to be the optimal condition of in vitro measuring of ammonia concentration. These high amounts of ammonia were ascribed to be most probably caused by the activation of glutaminase.

Study character:

Study funded by