Study type: Medical/biological study (experimental study)

Neoplastic transformation in C3H 10T(1/2) cells after exposure to 835.62 MHz FDMA and 847.74 MHz CDMA radiations. med./bio.

Published in: Radiat Res 2001; 155 (1) Pt 2: 239-247

Aim of study (acc. to author)

Aim of study was to determine if 835.62 MHz FDMA or 847.74 MHz CDMA radiations have any genotoxic effects that induce neoplastic transformations on C3H 10T1/2 cells in vitro.

Background/further details

To determine if exposure to FDMA or CDMA has any epigenetic effects that can promote neoplastic transformation, cells were also first exposed to 4.5 Gy of X-rays to induce the transformation process and then exposed to the above irradiations (SAR = 0.6 W/kg) for 42 days.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 835.62 MHz
Exposure duration: continuous for 7 or 42 days
Exposure 2: 847.74 MHz
Exposure duration: continuous for 7 or 42 days

Exposure 1

Main characteristics
Frequency 835.62 MHz
Type
Charakteristic
  • guided field
Exposure duration continuous for 7 or 42 days
Modulation
Modulation type cf. additional info
Additional info

FDMA

Exposure setup
Exposure source
Distance between exposed object and exposure source 29.2 cm
Chamber The Radial Transmission Line (RTL) irradiator and the shielded and temperature-controlled facility housing nine RTLs have been described and comprehensively characterised previously [Moros et al., 1999]. Each RTL was formed by two parallel metal plates, 4.3 cm apart, with a lightweight composite top plate hinged to the bottom aluminium plate (1/4" thick) providing mechanical support and equalising the temperatures of the flasks. The TEM waves travelling radially from the central conical antenna (10.2 cm radius) were terminated by an annulus of MW-absorbing foam backed by a perforated aluminium lamina. The radius of the entire parallel region from the antenna's center to the lamina was 53.3 cm.
Setup Sixteen sealed T-75 flasks, each containing 40 ml of culture medium, were positioned in the region around the antenna at a mean radial position of 29.2 cm, for a total of 1200 cm² of culture area per RTL, and maintained at 37.0 ± 0.3°C.
Sham exposure A sham exposure was conducted.
Additional info In some experiments, TPA was added to the culture medium when cells were placed in the incubators.
Parameters
Measurand Value Type Method Mass Remarks
SAR 0.6 W/kg mean measured - ± 0.3 W/kg

Exposure 2

Main characteristics
Frequency 847.74 MHz
Type
Charakteristic
  • guided field
Exposure duration continuous for 7 or 42 days
Modulation
Modulation type cf. additional info
Additional info

CDMA

Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
SAR 0.6 W/kg mean measured - ± 0.3 W/kg

Reference articles

  • Pickard WF et al. (2000): Experimental and numerical determination of SAR distributions within culture flasks in a dielectric loaded radial transmission line.
  • Moros EG et al. (1999): The radial transmission line as a broad-band shielded exposure system for microwave irradiation of large numbers of culture flasks.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

After both exposures (7 day RF exposure (835.62 MHz FDMA or 847.74 MHz CDMA) and 42 day exposure after X-ray irradiation) no statistically significant differences in the neoplastic transformation frequencies were observed.

Study character:

Study funded by

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