研究のタイプ: 医学/生物学の研究 (experimental study)

[835.62MHzのFDMAおよび847.74MHzのCDMA放射ばく露後のC3H10T(1/2)細胞における腫瘍性形質転換] med./bio.

Neoplastic transformation in C3H 10T(1/2) cells after exposure to 835.62 MHz FDMA and 847.74 MHz CDMA radiations.

掲載誌: Radiat Res 2001; 155 (1) Pt 2: 239-247

この研究は、インビトロでのC3H 10T(1/2)細胞の形質転換アッセイを用いて、携帯電話通信周波数範囲の無線周波電磁放射新生物性形質転換頻度に与える影響を調べた。実験に用いた周波数は、周波数分割多元接続FDMAの835.62 MHz符号分割多元接続CDMAの847.74 MHzである。まず、新生物性形質転換誘導するような遺伝毒性的影響を調べるために、37℃に保ったC3H 10T(1/2)細胞に、どちらかの周波数のRFばく露(どちらもSARは0.6 W/kg)または擬似ばく露を、7日間、同時刻に与えた。培地を交換した後、培養物をインキュベータに移し、7日毎に新鮮な増殖培地に替えた。42日後、細胞固定してギムザ染色し、形質転換病巣を記録した。次に、新生物性形質転換をプロモートするようなエピジェネティックな影響を調べるために、初めに細胞に4.5 GyのX線を照射して新生物性形質転換誘導した後、前述と同様の方法でRFばく露培地交換しながら42日間与えた。その結果、7日間ばく露およびX線ばく露後の42日間ばく露のどちらにおいても、インキュベータ対照群擬似ばく露群、どちらかの周波数のRFばく露群との間に形質転換頻度の有意差は観察されなかった、と報告している。

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研究目的(著者による)

Aim of study was to determine if 835.62 MHz FDMA or 847.74 MHz CDMA radiations have any genotoxic effects that induce neoplastic transformations on C3H 10T1/2 cells in vitro.

詳細情報

To determine if exposure to FDMA or CDMA has any epigenetic effects that can promote neoplastic transformation, cells were also first exposed to 4.5 Gy of X-rays to induce the transformation process and then exposed to the above irradiations (SAR = 0.6 W/kg) for 42 days.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 835.62 MHz
ばく露時間: continuous for 7 or 42 days
  • SAR: 0.6 W/kg mean (± 0.3 W/kg)
ばく露2: 847.74 MHz
ばく露時間: continuous for 7 or 42 days
  • SAR: 0.6 W/kg mean (± 0.3 W/kg)

ばく露1

主たる特性
周波数 835.62 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 7 or 42 days
Modulation
Modulation type cf. additional information
Additional information

FDMA

ばく露装置
ばく露の発生源/構造
Distance between exposed object and exposure source 29.2 cm
チャンバの詳細 The Radial Transmission Line (RTL) irradiator and the shielded and temperature-controlled facility housing nine RTLs have been described and comprehensively characterised previously [Moros et al., 1999]. Each RTL was formed by two parallel metal plates, 4.3 cm apart, with a lightweight composite top plate hinged to the bottom aluminium plate (1/4" thick) providing mechanical support and equalising the temperatures of the flasks. The TEM waves travelling radially from the central conical antenna (10.2 cm radius) were terminated by an annulus of MW-absorbing foam backed by a perforated aluminium lamina. The radius of the entire parallel region from the antenna's center to the lamina was 53.3 cm.
ばく露装置の詳細 Sixteen sealed T-75 flasks, each containing 40 ml of culture medium, were positioned in the region around the antenna at a mean radial position of 29.2 cm, for a total of 1200 cm² of culture area per RTL, and maintained at 37.0 ± 0.3°C.
Sham exposure A sham exposure was conducted.
Additional information In some experiments, TPA was added to the culture medium when cells were placed in the incubators.
パラメータ
測定量 種別 Method Mass 備考
SAR 0.6 W/kg mean 測定値 - ± 0.3 W/kg

ばく露2

主たる特性
周波数 847.74 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 7 or 42 days
Modulation
Modulation type cf. additional information
Additional information

CDMA

ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
SAR 0.6 W/kg mean 測定値 - ± 0.3 W/kg

Reference articles

  • Pickard WF et al. (2000): [誘電体装荷放射状送電線における培養瓶内のSAR分布の実験的および数的測定]
  • Moros EG et al. (1999): [大量の培養瓶へのマイクロ波照射用広帯域遮蔽ばく露装置としての放射状送電線]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

After both exposures (7 day RF exposure (835.62 MHz FDMA or 847.74 MHz CDMA) and 42 day exposure after X-ray irradiation) no statistically significant differences in the neoplastic transformation frequencies were observed.

研究の種別:

研究助成

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