Study type: Medical/biological study (experimental study)

Large granular lymphocytic (LGL) leukemia in rats exposed to intermittent 60 Hz magnetic fields. med./bio.

Published in: Bioelectromagnetics 2001; 22 (3): 185-193

Aim of study (acc. to author)

To investigate the effects of a 60 Hz magnetic field exposure on progression of leukemia in the LGL-rat model. The LGL (large granular lymphocytic) leukemia model is used to induce leukemia in young, male rats within a relatively short period of time by engrafting spleen cells from aged leukemic rats. Main objectives were: a) to replicate a previous study, using a lower number of intraperitoneally injected LGL leukemic spleen cells (i.e. 2.2x106 instead of 2.2x107 cells), and b) to determine if effects of an intermitted 60 Hz magnetic field (3 min on/off interval) are distinguishable from those of a continuous magnetic field exposure.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: repeated daily exposure, 20h/day, 7days/week for 16 weeks
Exposure 2: 60 Hz
Exposure duration: intermittent, 3 min on/off, 20 h/day, 7days/week for 16 weeks
intermittend (3 min on/off interval)

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Waveform
Polarization
Exposure duration repeated daily exposure, 20h/day, 7days/week for 16 weeks
Exposure setup
Exposure source
Chamber polyethylen cages
Additional info The exposure system consisted of four units with six 1.05 x 3.6 m cage support shelves spaced 30 cm apart. Within each unit, the shelves are arranged vertically into two groups of three, with each group surrounded by coils. Each exposure system had four tiers for holding rat cages with each tier accommodating four polycarbonate modules of six individual rat cages on each side.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT mean measured - -

Exposure 2

Main characteristics
Frequency 60 Hz
Type
Waveform
Polarization
Exposure duration intermittent, 3 min on/off, 20 h/day, 7days/week for 16 weeks
Additional info intermittend (3 min on/off interval)
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT mean measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

A total of 72 animal (divided into 4 groups, 18 animals each) were used in this study. No significant differences in either survival time or body weight could be found between the exposed and the unexposed animal. Furthermore, no consistant differences on hematological pattern could be demonstrated between continuously or intermittently exposed rats.

Study character:

Study funded by

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