To investigate the hypothesis whether oxidative stress may play a role in the pathophysiology of microwave-induced circulatory failure. The question was examined by probing organs for 3-nitrotyrosine (3-NT), a marker of oxidative stress.
Modulation type: CW
Exposure duration: until 38°C (15 ± 3 min), 39°C (24 ± 1.5 min), 40°C (33.7 ± 1.9 min) or 41°C (44.2 ± 1.8 min) or until blood pressure 75mmHg (60.3 ± 2.4 min)
|Distance between exposed object and exposure source||1.1 m|
|Chamber||RF-shielded anechoic chamber maintained at 27°C, 20% humidity.|
|Setup||The animal was centered along the boresight and exposed in a Plexiglass holder in a H-orientation (long axis of the body parallel to magnetic field)|
|Additional info||Animals were divided into 7 groups (n=8/group): i) animals were placed on the exposure platform but were not exposed and were heated with water-perfused heating pad until 37°C was reached ii)-v) Animals were exposed to microwave until 38, 39, 40 or 41°C were reached vi) Animals were exposed until 75 mmHg was reached vii) animals were anesthetized and instrumented but were not exposed and the temperature was maintained at 37°C and monitored for 55 min as a time contol.|
The findings suggest that oxidative stress occurs in many organs during microwave heating. Because nitration occurs after microwave exposures that are not associated with circulatory collapse, systemic oxidative stress, as evidenced by tissue accumulation of 3-NT, is not correlated with circulatory failure in this model of shock.