Study type: Medical/biological study (experimental study)

Effects of millimeter-wave radiation on monolayer cell cultures. I. Design and validation of a novel exposure system. med./bio.

Published in: Bioelectromagnetics 1981; 2 (2): 123-140

Aim of study (acc. to author)

To establish a novel in vitro millimeter wave exposure system for monolayer cell cultures, which allows seeking for athermal biological effects under controlled temperature conditions.

Background/further details

As an indicator for possible biological effects the incorporation of radioactive labeled 3H-uridine and 3H-methionine into RNA and protein, respectively, was investigated by measuring of the optical densities of autoradiographes.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 65–75 GHz
Exposure duration: continuous
Exposure 2: 42–48 GHz
Exposure duration: continuous for 1 h
Exposure 3: 37–42 GHz
Exposure duration: continuous for 1 h

Exposure 1

Main characteristics
Frequency 65–75 GHz
Type
Exposure duration continuous
Exposure setup
Exposure source
Setup cells grown on microwave-transparent polystyrene coverslips, placed directly on the open end of the waveguide
Parameters
Measurand Value Type Method Mass Remarks
power density 450 mW/cm² average over time measured - under the surface of the petri dish

Exposure 2

Main characteristics
Frequency 42–48 GHz
Type
Exposure duration continuous for 1 h
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
power 180 mW - - - -

Exposure 3

Main characteristics
Frequency 37–42 GHz
Type
Exposure duration continuous for 1 h
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
power density 320 mW/cm² average over time measured - under the surface of the petri dish

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

Microwave-induced changes on RNA or protein synthesis did not occur when the temperature of cell cultures where set to 37°C by recirculation of the medium. In contrast, the cultures were affected (in such a way of marked heating, decline of macromolecular synthesis, and destruction of the monolayer) when recirculating and temperature control were shut down.

Study character:

Study funded by

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