The effects of exposure to a 50 Hz magnetic field on the preintegrative steps of the replication of a murine retrovirus responsible for the development of leukemia/lymphoma should be investigated in infected T lymphocytes and B lymphocytes.
T lymphocytes and B lymphocytes were isolated from peripheral blood from 8-weeks old male Swiss mice and infected with the Moloney Murine Leukemia virus. Cells were divided into the following groups: 1) exposure of T lymphocytes to the 50 Hz magnetic field, 2) T lymphocytes control group (placed in another incubator), 3) T lymphocytes sham exposure group, 4) exposure of B lymphocytes to the 50 Hz magnetic field, 5) B lymphocytes control group, 6) T lymphocytes sham exposure group.
Cells were exposed during 3 days of mitogenic stimulation before infection. An additional exposure period of 90 min after infection was applied for measuring the effects of magnetic field exposure on virus entry. For the detection of the effects of exposure on the further steps of viral replication, cultures were exposed for an additional period of 24 h after viral infection.
Exposure duration: continuous for 3 to 4 days
|Setup||two coils superimposed on a metalic frame place inside a CO2 incubator; the two coils comprised 560 rounds of copper wire with a cross-sectional area of 2 mm2; the direction of the applied field in the incubator was horizontal and directed to the North; cultures were incubated at 37°C in an atmosphere of 5% CO2|
|Sham exposure||A sham exposure was conducted.|
|Additional info||in the sham exposure, the electrical connections were alternated, resulting in zero magnetic induction|
|magnetic flux density||1 mT||-||measured||-||-|
No significant differences were found between exposed cells and the control groups.
The authors conclude that no evidence was found for an effect of exposure to 50 Hz magnetic field on the preintegrative steps of replication of a murine retrovirus in infected T lymphocytes and B lymphocytes.