＜目的＞電磁界がMo-MLV（Moloney Murine Leukemia virus）複製の初期の過程に与える影響を評価する。 ＜方法＞Mo-MLVは慢性的に感染しているA9細胞から産生された。曝露コイルはインキュベーター中にセットされ、背景磁界は0.5microTであった。T、Bリンパ球は正弦波磁界（50Hz、1mT）に、感染前のマイトジェン刺激中3日間曝露され、感染後は、ウィルスが侵入するときの磁界の影響を見るために、さらに90分間曝露された。 ＜結果）PCR実験によるGag1/2増幅とU3/Gag3増幅は、micro g DNAあたりのウィルスコピー数では曝露群と対照群の間に差異はなかった。T、Bリンパ球のウィルスの環状DNA量にも、対照群と曝露群に統計的有意差はなかった。従って、白血病やリンパ腫の発生に寄与するネズミのレトロウィルスの複製の前組込過程は、電磁界の曝露によって影響を受けないことが示された。
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The effects of exposure to a 50 Hz magnetic field on the preintegrative steps of the replication of a murine retrovirus responsible for the development of leukemia/lymphoma should be investigated in infected T lymphocytes and B lymphocytes.
T lymphocytes and B lymphocytes were isolated from peripheral blood from 8-weeks old male Swiss mice and infected with the Moloney Murine Leukemia virus. Cells were divided into the following groups: 1) exposure of T lymphocytes to the 50 Hz magnetic field, 2) T lymphocytes control group (placed in another incubator), 3) T lymphocytes sham exposure group, 4) exposure of B lymphocytes to the 50 Hz magnetic field, 5) B lymphocytes control group, 6) T lymphocytes sham exposure group.
Cells were exposed during 3 days of mitogenic stimulation before infection. An additional exposure period of 90 min after infection was applied for measuring the effects of magnetic field exposure on virus entry. For the detection of the effects of exposure on the further steps of viral replication, cultures were exposed for an additional period of 24 h after viral infection.
ばく露時間: continuous for 3 to 4 days
|ばく露時間||continuous for 3 to 4 days|
|ばく露装置の詳細||two coils superimposed on a metalic frame place inside a CO2 incubator; the two coils comprised 560 rounds of copper wire with a cross-sectional area of 2 mm2; the direction of the applied field in the incubator was horizontal and directed to the North; cultures were incubated at 37°C in an atmosphere of 5% CO2|
|Sham exposure||A sham exposure was conducted.|
|Additional information||in the sham exposure, the electrical connections were alternated, resulting in zero magnetic induction|
No significant differences were found between exposed cells and the control groups.
The authors conclude that no evidence was found for an effect of exposure to 50 Hz magnetic field on the preintegrative steps of replication of a murine retrovirus in infected T lymphocytes and B lymphocytes.