Study type: Medical/biological study (experimental study)

Physiologic aging of mature porcine erythrocytes: effects of various metabolites, antimetabolites, and physical stressors med./bio.

By: Kiel JL, Erwin DN
Published in: Am J Vet Res 1986; 47 (10): 2155-2160
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Aim of study (acc. to author)

The purpose of this study was to evaluate the hemolytic response of erythrocytes on heating, achieved by microwave irradiation, hot air, or hot-water bath, in the presence of different chemical substances in vitro.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 2.45 GHz
Modulation type: CW
Exposure duration: 15 min

Exposure 1

Main characteristics
Frequency 2.45 GHz
Type
Waveform
Exposure duration 15 min
Modulation
Modulation type CW
Exposure setup
Exposure source
  • not specified
Setup polypropylene centrifuge tubes
Parameters
Measurand Value Type Method Mass Remarks
SAR 91 mW/g mean - - +/- 47 W/Kg.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Heating erythrocytes to 43°C for 10 min with microwaves or hot air did not significantly increase hemolysis, compared with hemolysis at 4°C. Pyruvate or lactate did not affect the erythrocytes under these conditions. Hemolysis of cells coated with concanavalin A-luminol-bovine serum albumin and heated to 43°C for 10 min by use of microwaves or hot air was significantly greater than that of controls at 4°C. L-mimosine did not affect thermal hemolysis. 3-amino-L-tyrosin suppressed thermal hemolysis to that of the controls.

Study character:

Study funded by

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