The purpose of this study was to evaluate the hemolytic response of erythrocytes on heating, achieved by microwave irradiation, hot air, or hot-water bath, in the presence of different chemical substances in vitro.
|Setup||polypropylene centrifuge tubes|
Heating erythrocytes to 43°C for 10 min with microwaves or hot air did not significantly increase hemolysis, compared with hemolysis at 4°C. Pyruvate or lactate did not affect the erythrocytes under these conditions. Hemolysis of cells coated with concanavalin A-luminol-bovine serum albumin and heated to 43°C for 10 min by use of microwaves or hot air was significantly greater than that of controls at 4°C. L-mimosine did not affect thermal hemolysis. 3-amino-L-tyrosin suppressed thermal hemolysis to that of the controls.