Study type: Medical/biological study (experimental study)

DNA damage in frog erythrocytes after in vitro exposure to a high peak-power pulsed electromagnetic field. med./bio.

Published in: Mutat Res Genet Toxicol Environ Mutagen 2004; 558 (1-2): 27-34

Aim of study (acc. to author)

To study possible genotoxic effects of high peak-power pulsed electromagnetic fields on erythrocytes of the frog Xenopus laevis (South African male clawd toads).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 8.8 GHz
Modulation type: pulsed
Exposure duration: continuous for 40 min

General information

Ionization radiation exposure were conducted with the cells exposed to 0, 50, 100 and 200 cGy from 60Co gamma ray source

Exposure 1

Main characteristics
Frequency 8.8 GHz
Charakteristic
  • guided field
Exposure duration continuous for 40 min
Modulation
Modulation type pulsed
Pulse width 180 ns
Repetition frequency 50 Hz
Exposure setup
Exposure source
Setup Fifty microliters of cell suspension (25±1°C at the beginning and 29±1°C at the end of the exposure) were exposed in special plastic cuvettes of cylindrical shape placed inside a rectangular wave guide at a distance of 25 to 30 mm from the wave guide.
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 1,420 kV/m maximum - - -
SAR 1.6 W/g - calculated cf. remarks calculated from temperature kinetic measurements
SAR 300,000 kW/kg peak value - - -

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The temperature rise in the blood samples was 3.5 +/- 0.1°C. The results show that the increase in DNA damage after exposure of erythrocytes to high peak-power pulsed electromagnetic fields was induced by the rise in temperature in the irradiated cell suspension. This was confirmed in experiments in which blood samples were incubated for 40 min under the corresponding temperature conditions. The data allow the authors to conclude that high peak-power pulsed electromagnetic field-exposure at the given modality did not cause any athermal genotoxic effect on frog erythrocytes in vitro.

Study character:

Study funded by

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