|Exposure duration||continuous for 40 min|
|Pulse width||180 ns|
|Repetition frequency||50 Hz|
|Setup||Fifty microliters of cell suspension (25±1°C at the beginning and 29±1°C at the end of the exposure) were exposed in special plastic cuvettes of cylindrical shape placed inside a rectangular wave guide at a distance of 25 to 30 mm from the wave guide.|
|Sham exposure||A sham exposure was conducted.|
The temperature rise in the blood samples was 3.5 +/- 0.1°C. The results show that the increase in DNA damage after exposure of erythrocytes to high peak-power pulsed electromagnetic fields was induced by the rise in temperature in the irradiated cell suspension. This was confirmed in experiments in which blood samples were incubated for 40 min under the corresponding temperature conditions. The data allow the authors to conclude that high peak-power pulsed electromagnetic field-exposure at the given modality did not cause any athermal genotoxic effect on frog erythrocytes in vitro.