この研究は、高ピーク電力のパルス化電磁界（HPPP EMF：8.8GHz、パルス幅180ナノ秒、ピーク電力65 kW、繰り返し周波数50 Hz）がカエルの赤血球に対して遺伝毒性的影響を示すか否かを調べた。DNA 単鎖切断とアルカリ不安定損傷を評価する感受性の高い方法であるアルカリコメットアッセイを用いた。血液サンプルに、方形導波管内で40分間のHPPPEMFばく露を与えた。血液サンプルのSARは1.6 W/kgで、温度上昇は安定状態で3.5±0.1℃であった。その結果、HPPPEMFばく露は、非熱的な遺伝毒性作用を示さなかった､と報告している。
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To study possible genotoxic effects of high peak-power pulsed electromagnetic fields on erythrocytes of the frog Xenopus laevis (South African male clawd toads).
Ionization radiation exposure were conducted with the cells exposed to 0, 50, 100 and 200 cGy from 60Co gamma ray source
|ばく露時間||continuous for 40 min|
|Pulse width||180 ns|
|Repetition frequency||50 Hz|
|ばく露装置の詳細||Fifty microliters of cell suspension (25±1°C at the beginning and 29±1°C at the end of the exposure) were exposed in special plastic cuvettes of cylindrical shape placed inside a rectangular wave guide at a distance of 25 to 30 mm from the wave guide.|
|Sham exposure||A sham exposure was conducted.|
The temperature rise in the blood samples was 3.5 +/- 0.1°C. The results show that the increase in DNA damage after exposure of erythrocytes to high peak-power pulsed electromagnetic fields was induced by the rise in temperature in the irradiated cell suspension. This was confirmed in experiments in which blood samples were incubated for 40 min under the corresponding temperature conditions. The data allow the authors to conclude that high peak-power pulsed electromagnetic field-exposure at the given modality did not cause any athermal genotoxic effect on frog erythrocytes in vitro.