研究のタイプ: 医学/生物学の研究 (experimental study)

[高ピーク電力パルス電磁界にインビトロばく露した後のカエル赤血球のDNA損傷] med./bio.

DNA damage in frog erythrocytes after in vitro exposure to a high peak-power pulsed electromagnetic field.

掲載誌: Mutat Res Genet Toxicol Environ Mutagen 2004; 558 (1-2): 27-34

この研究は、高ピーク電力パルス電磁界(HPPP EMF:8.8GHz、パルス幅180ナノ秒、ピーク電力65 kW、繰り返し周波数50 Hz)がカエルの赤血球に対して遺伝毒性的影響を示すか否かを調べた。DNA 単鎖切断とアルカリ不安損傷を評価する感受性の高い方法であるアルカリコメットアッセイを用いた。血液サンプルに、方形導波管内で40分間のHPPPEMFばく露を与えた。血液サンプルのSARは1.6 W/kgで、温度上昇は安定状態で3.5±0.1℃であった。その結果、HPPPEMFばく露は、非熱的遺伝毒性作用を示さなかった、と報告している。

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研究目的(著者による)

To study possible genotoxic effects of high peak-power pulsed electromagnetic fields on erythrocytes of the frog Xenopus laevis (South African male clawd toads).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 8.8 GHz
Modulation type: pulsed
ばく露時間: continuous for 40 min
  • 電界強度: 1,420 kV/m maximum
  • SAR: 1.6 W/g (calculated from temperature kinetic measurements)
  • SAR: 300,000 kW/kg peak

General information

Ionization radiation exposure were conducted with the cells exposed to 0, 50, 100 and 200 cGy from 60Co gamma ray source

ばく露1

主たる特性
周波数 8.8 GHz
特性
  • guided field
ばく露時間 continuous for 40 min
Modulation
Modulation type pulsed
Pulse width 180 ns
Repetition frequency 50 Hz
ばく露装置
ばく露の発生源/構造
  • rectangular waveguide
ばく露装置の詳細 Fifty microliters of cell suspension (25±1°C at the beginning and 29±1°C at the end of the exposure) were exposed in special plastic cuvettes of cylindrical shape placed inside a rectangular wave guide at a distance of 25 to 30 mm from the wave guide.
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電界強度 1,420 kV/m maximum - - -
SAR 1.6 W/g - 計算値 cf. remarks calculated from temperature kinetic measurements
SAR 300,000 kW/kg peak - - -

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The temperature rise in the blood samples was 3.5 +/- 0.1°C. The results show that the increase in DNA damage after exposure of erythrocytes to high peak-power pulsed electromagnetic fields was induced by the rise in temperature in the irradiated cell suspension. This was confirmed in experiments in which blood samples were incubated for 40 min under the corresponding temperature conditions. The data allow the authors to conclude that high peak-power pulsed electromagnetic field-exposure at the given modality did not cause any athermal genotoxic effect on frog erythrocytes in vitro.

研究の種別:

研究助成

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