Study type: Medical/biological study (experimental study)

Acute effects of low-frequency electromagnetic fields on leukocyte-endothelial interactions in vivo. med./bio.

Published in: In Vivo 2004; 18 (2): 125-132

Aim of study (acc. to author)

To clarify the effect of extremely low frequency electromagnetic field on microcirculation, acute effects on leukocyte-endothelium interactions resulting from extremely low frequency electromagnetic field exposure were examined in conscious mice.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 30 min
Exposure 2: 50 Hz
Exposure duration: 30 min
Exposure 3: 50 Hz
Exposure duration: 30 min

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 30 min
Exposure setup
Exposure source
Setup electromagnet consisting of a C-shaped steel bar with four blocks of exposure coils; a non metallic dorsal skinfold chamber was implanted in the animal; animals placed in an acrylic resin tube of 26 mm (inner diameter) which was fixed onto a non metallic microscope stage and then placed within the electromagnet
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 3 mT unspecified measured - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 30 min
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 10 mT unspecified measured - -

Exposure 3

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 30 min
Additional info Exposure and sham exposure groups consisted of 10 to 11 animals each.
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 30 mT unspecified measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • before exposure
  • after exposure

Main outcome of study (acc. to author)

Following exposure, the mean velocity of free flowing leukocytes in the center stream of venules showed no distinct changes. However, at a flux density of 30 mT the number of adherent leukocytes increased significantly following exposure; no significant differences in adherent cell numbers were found in the 3 mT and 10 mT exposed mice or sham exposed controls comparing pre- and post-exposure periods.
These data demonstrated that high magnetic flux densities of extremely low frequency electromagnetic field had an effect on leukocyte-endothelium interactions. The results indicate that a threshold level for this phenomenon exists in the range of 10-30 mT under these experimental conditions.

Study character:

Study funded by

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