To clarify the effect of extremely low frequency electromagnetic field on microcirculation, acute effects on leukocyte-endothelium interactions resulting from extremely low frequency electromagnetic field exposure were examined in conscious mice.
|Setup||electromagnet consisting of a C-shaped steel bar with four blocks of exposure coils; a non metallic dorsal skinfold chamber was implanted in the animal; animals placed in an acrylic resin tube of 26 mm (inner diameter) which was fixed onto a non metallic microscope stage and then placed within the electromagnet|
|Sham exposure||A sham exposure was conducted.|
|magnetic flux density||3 mT||unspecified||measured||-||-|
|magnetic flux density||10 mT||unspecified||measured||-||-|
|magnetic flux density||30 mT||unspecified||measured||-||-|
Following exposure, the mean velocity of free flowing leukocytes in the center stream of venules showed no distinct changes. However, at a flux density of 30 mT the number of adherent leukocytes increased significantly following exposure; no significant differences in adherent cell numbers were found in the 3 mT and 10 mT exposed mice or sham exposed controls comparing pre- and post-exposure periods.
These data demonstrated that high magnetic flux densities of extremely low frequency electromagnetic field had an effect on leukocyte-endothelium interactions. The results indicate that a threshold level for this phenomenon exists in the range of 10-30 mT under these experimental conditions.