Study type: Medical/biological study (experimental study)

Lymphoblastoid transformation of lymphocytes in vitro after microwave irradiation. med./bio.

Published in: Nature 1967; 214 (83): 102-103

Aim of study (acc. to author)

To study the influence of microwaves on mitosis. Lymphoblastoid transformation of lymphocytes was found by chance.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 3 GHz
Exposure duration: repeated daily exposure, 4 h daily for 3 days
Exposure 2: 3 GHz
Exposure duration: repeated daily exposure, 4 h daily for 5 days
Exposure 3: 3 GHz
Exposure duration: repeated daily exposure, 15 min daily for 3 days
Exposure 4: 3 GHz
Exposure duration: repeated daily exposure, 15 min daily for 5 days

Exposure 1

Main characteristics
Frequency 3 GHz
Exposure duration repeated daily exposure, 4 h daily for 3 days
Modulation
Modulation type unspecified
Exposure setup
Exposure source
Setup The test tubes containing the cultures were placed in a Plexiglass thermostat maintained at 37°C.
Parameters
Measurand Value Type Method Mass Remarks
power density 70 W/m² unspecified unspecified - -

Exposure 2

Main characteristics
Frequency 3 GHz
Exposure duration repeated daily exposure, 4 h daily for 5 days
Modulation
Modulation type unspecified
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
power density 70 W/m² unspecified unspecified - -

Exposure 3

Main characteristics
Frequency 3 GHz
Exposure duration repeated daily exposure, 15 min daily for 3 days
Modulation
Modulation type unspecified
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
power density 200 W/m² unspecified unspecified - -

Exposure 4

Main characteristics
Frequency 3 GHz
Exposure duration repeated daily exposure, 15 min daily for 5 days
Modulation
Modulation type unspecified
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
power density 200 W/m² unspecified unspecified - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

After incubation for 3 days in cultures exposed at a power density of 7 mW/cm² the percentage of transformed cells was twice as high as in control cultures. Similar data were obtained in cultures exposed at a power density of 20 mW/cm². After incubation of 3 days a slight increase in the percentage of macrophages was noted. In some cases sporadic mitoses were observed. After incubation for 5 days similar changes were revealed at both power densities. The percentage of transformed lymphocytes was five times as high as that in control cultures. The percentage of macrophages was also high, and there was 2-3% of mitoses. Fragmented nuclei could be found among the transformed cells.
The data of control experiments showed that the effetcs can be attributed to the effect of microwave irradiation and not to any effect of heating.

Study character:

Study funded by

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