Study type: Medical/biological study (experimental study)

Millimeter wave-induced suppression of B16 F10 melanoma growth in mice: involvement of endogenous opioids med./bio.

By: Radzievsky AA, Gordiienko OV, Szabo I, Alekseev SI, Ziskin MC
Published in: Bioelectromagnetics 2004; 25 (6): 466-473
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Aim of study (acc. to author)

To evaluate quantitatively the ability of millimeter wave treatment to influence tumor growth and to assess whether endogenous opioids are involved. The experimental mouse model of B16 F10 melanoma subcutaneous growth was used (melanoma growth was modeled by subcutaneous injection into the left flank of B16F10 melanoma cells). Naloxone (1 mg/kg, 30 min prior to millimeter wave treatment) was used as a nonspecific blocker of opioid receptors.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 61.22 GHz
Exposure duration: repeated daily exposure, 15 min/day for 5 days
Exposure 2: 61.22 GHz
Exposure duration: 15 min

Exposure 1

Main characteristics
Frequency 61.22 GHz
Charakteristic
Exposure duration repeated daily exposure, 15 min/day for 5 days
Modulation
Modulation type unspecified
Exposure setup
Exposure source
Chamber Shielded chamber made of 0.5 inch thick low carbon steel sheets
Setup Mice (not anesthetized) were restrained in a plastic tube with the nose uncovered and the tail placed outside the restrainer. The nose was located in the center of the antenna`s aperture (1.5 cm x 1.5 cm)
Parameters
Measurand Value Type Method Mass Remarks
power density 133 W/m² mean calculated - -

Exposure 2

Main characteristics
Frequency 61.22 GHz
Charakteristic
Exposure duration 15 min
Modulation
Modulation type unspecified
Exposure setup
Exposure source
Chamber Shielded chamber made of 0.5 inch thick low carbon steel sheets
Setup Five mice were anesthetized and exposed without being restrained for obtaining thermographic images
Parameters
Measurand Value Type Method Mass Remarks
power density 133 W/m² mean calculated - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

Five daily millimeter wave irradiations, if applied starting at the fifth day following B16 melanoma cell injection, suppressed subcutaneous tumor growth. Pretreatment with naloxone completely abolished the millimeter wave treatment-induced suppression of melanoma growth. The same course of 5 millimeter wave treatments, if started on day 1 or day 10 following tumor inoculations, was ineffective. The authors concluded that millimeter wave treatment has an anticancer therapeutic potential and that endogenous opioids are involved in millimeter wave treatment-induced suppression of melanoma growth.

Study character:

Study funded by

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