Study type: Medical/biological study (experimental study)

Neuronal outgrowth of PC-12 cells after combined treatment with nerve growth factor and a magnetic field: Influence of the induced electric field strength med./bio.

By: Schimmelpfeng J, Weibezahn KF, Dertinger H
Published in: Bioelectromagnetics 2005; 26 (1): 74-77
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Aim of study (acc. to author)

To investigate the effects of a combined treatment with nerve growth factor (NGF) and a magnetic field (100 µT/ 50 Hz) on neuronal growth of rat PC-12 cells in vitro.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 8 h/day for 2 to 6 days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 8 h/day for 2 to 6 days
Exposure setup
Exposure source
Setup 2 round coils with a radius of 6.5 cm, 6 cm apart, placed in an incubator; one culture dish placed inside the coils system, one outside; magnetic field vertical and perpendicular to the culture dishes
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 100 µT effective value calculated - -
electric field strength 251 µV/m peak value calculated - in the outer compartment (r = 1.6 - 2.55 cm)
electric field strength 400 µV/m peak value calculated - in the outer compartment (r = 1.6 - 2.55 cm)
electric field strength 135 µV/m peak value calculated - in the inner compartment (r = 0 - 0.85 cm)

Reference articles

  • Aaron RK et al. (1999): Power frequency fields promote cell differentiation coincident with an increase in transforming growth factor-beta(1) expression

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Magnetic field exposure (100 µT/50 Hz) did not lead to statistically significant alterations of neuronal growth, induced by nerve growth factor, in rat PC-12 cells in vitro.

Study character:

Study funded by

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