Medical/biological study (experimental study)

Effects of ELF magnetic fields on protein expression profile of human breast cancer cell MCF7 med./bio.

By: Li H, Zeng Q, Weng Y, Lu D, Jiang H, Xu Z

Published in: Sci China C Life Sci 2005; 48 (5): 506-514

Exposure	Parameters
Exposure 1: 50 Hz Exposure duration: continuous for 24 hours	magnetic flux density: 0.4 mT

Exposure source	Helmholtz coils
Setup	The exposure system consisted of three groups of Helmholtz coils, two power regulators and a set of CO₂ incubators. The magnetic fields were generated from line current . Cell culture dishes were placed coaxially with the centerline in the central area of the coils. Sham exposed cells as control were placed in an identical incubator without magnetic fields.
Additional info	The AC background field was 1-2 µT and the static magnetic field was 18.5 µT, with a 14.1 µT horizontal and a 12.0 µT vertical component.

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	0.4 mT	-	measured	-	-

Magnetic flux density was determined using a power frequency field meter.

Exposed system:

Investigated system:

Time of investigation:

Six protein spots have been statistically significantly changed (their expression levels were altered at least 5 fold up or down) compared with sham-exposed group. 19 protein spots were only detected in exposure group while 19 ones were missing.
Three proteins of the six differential protein spots were identified as "RNA binding protein regulatory subunit", "Proteasome subunit beta type 7 precursor" and "Translationally Controlled Tumor Protein" (TCTP).
The data showed that 50 Hz extremely low frequency magnetic field changes the protein profile of MCF7 cells and may affect many physiological functions of normal cells. Two-dimensional gel electrophoresis coupled with mass spectrometry is a promising approach to elucidating cellular effects of electromagnetic fields.

Study character:

Sanie-Jahromi F et al. (2016): Effects of extremely low frequency electromagnetic field and cisplatin on mRNA levels of some DNA repair genes
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