Study type: Medical/biological study (experimental study)

Effects of ELF magnetic fields on protein expression profile of human breast cancer cell MCF7. med./bio.

Published in: Sci China C Life Sci 2005; 48 (5): 506-514

Aim of study (acc. to author)

A proteomics approach was employed to study the changes of protein expression profile induced by extremely low frequency magnetic field (50 Hz) in human breast cancer cell line MCF7, in order to determine extremely low frequency magnetic field-responsive proteins.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 24 hours

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 24 hours
Exposure setup
Exposure source
Setup The exposure system consisted of three groups of Helmholtz coils, two power regulators and a set of CO2 incubators. The magnetic fields were generated from line current . Cell culture dishes were placed coaxially with the centerline in the central area of the coils. Sham exposed cells as control were placed in an identical incubator without magnetic fields.
Additional info The AC background field was 1-2 µT and the static magnetic field was 18.5 µT, with a 14.1 µT horizontal and a 12.0 µT vertical component.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.4 mT - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Six protein spots have been statistically significantly changed (their expression levels were altered at least 5 fold up or down) compared with sham-exposed group. 19 protein spots were only detected in exposure group while 19 ones were missing.
Three proteins of the six differential protein spots were identified as "RNA binding protein regulatory subunit", "Proteasome subunit beta type 7 precursor" and "Translationally Controlled Tumor Protein" (TCTP).
The data showed that 50 Hz extremely low frequency magnetic field changes the protein profile of MCF7 cells and may affect many physiological functions of normal cells. Two-dimensional gel electrophoresis coupled with mass spectrometry is a promising approach to elucidating cellular effects of electromagnetic fields.

Study character:

Study funded by

Related articles