A proteomics approach was employed to study the changes of protein expression profile induced by extremely low frequency magnetic field (50 Hz) in human breast cancer cell line MCF7, in order to determine extremely low frequency magnetic field-responsive proteins.
Exposure duration: continuous for 24 hours
|Setup||The exposure system consisted of three groups of Helmholtz coils, two power regulators and a set of CO2 incubators. The magnetic fields were generated from line current . Cell culture dishes were placed coaxially with the centerline in the central area of the coils. Sham exposed cells as control were placed in an identical incubator without magnetic fields.|
|Additional info||The AC background field was 1-2 µT and the static magnetic field was 18.5 µT, with a 14.1 µT horizontal and a 12.0 µT vertical component.|
|magnetic flux density||0.4 mT||-||measured||-||-|
Six protein spots have been statistically significantly changed (their expression levels were altered at least 5 fold up or down) compared with sham-exposed group. 19 protein spots were only detected in exposure group while 19 ones were missing.
Three proteins of the six differential protein spots were identified as "RNA binding protein regulatory subunit", "Proteasome subunit beta type 7 precursor" and "Translationally Controlled Tumor Protein" (TCTP).
The data showed that 50 Hz extremely low frequency magnetic field changes the protein profile of MCF7 cells and may affect many physiological functions of normal cells. Two-dimensional gel electrophoresis coupled with mass spectrometry is a promising approach to elucidating cellular effects of electromagnetic fields.