Study type: Medical/biological study (experimental study)

Effect of ELF-EMF Exposure on Human Neuroblastoma Cell Line: a Proteomics Analysis. med./bio.

Published in: Iran J Cancer Prev 2014; 7 (1): 22-27

Aim of study (acc. to author)

The potential effects of an in vitro exposure of human neuroblastoma cells to a 50 Hz magnetic field on the protein expression should be investigated.

Background/further details

For protein expression analysis, each sample (protein extracts from control and exposed cells) was loaded on three gels and investigated.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 3 h

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 3 h
Exposure setup
Exposure source
Setup Helmholtz coils installed inside an incubator.
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2 mT - - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Morphological differences were spotted between exposed cells and control during microscopic analysis (no details described). Protein expression analysis showed a significant up-regulation or down-regulation of 189 proteins in the samples of exposed cells compared to control.
The authors conclude that an in vitro exposure of human neuroblastoma cells to a 50 Hz magnetic field has effects on the protein expression which in turn affects cell morphology. However, more studies are necessary to identify the proteins affected and to clarify further relationships.

Study character:

Study funded by

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