Study type: Medical/biological study (experimental study)

Real-time measurement of cytosolic free calcium concentration in Jurkat cells during ELF magnetic field exposure and evaluation of the role of cell cycle. med./bio.

Published in: Bioelectromagnetics 2006; 27 (5): 354-364

Aim of study (acc. to author)

To study the biological conditions under which extremely low frequency magnetic field exposure might alter cytosolic free calcium content.

Background/further details

Cytosolic free calcium was measured during exposureto extremely low frequency magnetic field in Jurkat E6.1 cells synchronized to different phases of the cell cycle (G0/G1, S, and G2-M- phases). In each experiment, the dynamics of cytosolic free calcium content during magnetic field exposure was monitored at three different stages of activation by anti-CD3: resting or basal cytosolic free calcium content prior to anti-CD3 activation, the initial and sustained free calcium response after anti-CD3 application.
Extremely low frequency magnetic fields have been reported to alter a number of cell signaling pathways, including those involved in cell proliferation, cell differentiation and apoptosis where cytosolic free calcium plays an important role.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: continuous from 300 s till 3600 s

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration continuous from 300 s till 3600 s
Additional info Samples exposed to a combined sinusoidal and static MF.
Exposure setup
Exposure source
Setup Cultures were kept in a Mu-metal box (33 x 38 x 20 cm) which was kept in an incubator maintained at 37°C.
Additional info Cells were subjected to zero MF from 300 s and then subjected to either zero MF or AC+DC MF.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 78.1 µT - unspecified - ± 0.5 µT; Static MF
magnetic flux density 100 µT unspecified unspecified - ± 0.5 µT; sinusoidal MF

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure

Main outcome of study (acc. to author)

The data indicate (i) that the extremely low frequency magnetic field increased cytosolic free calcium content during an antibody-induced signaling event, (ii) that the extremely low frequency magnetic field effect did not depend to a large degree on cell cycle, and (iii) that a field-related change in cytosolic free calcium signaling appeared to correlate with features in the cytosolic free calcium dynamics.

Study character:

Study funded by

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