Study type: Medical/biological study (experimental study)

Cancer promotion in a mouse-skin model by a 60-Hz magnetic field: II. Tumor development and immune response. med./bio.

Published in: Bioelectromagnetics 1991; 12 (5): 273-287

Aim of study (acc. to author)

To investigate the effects of a 60 Hz magnetic field exposure in the SENCAR mouse model.

Background/further details

DMBA and TPA were used for the chemical induction of skin tumor. Furthermore the activity of natural killer cells (NK cells) in spleen and blood were examined. A total of 160 animals were used in this study (5 groups/32 animals each). All animals received a single subcarcinogenic administration of DMBA (10 nmol). Group A (sham-exposed) and group B (daily exposed) were not treated with TPA, whereas group C (sham-exposed) and group D (exposed five days/week) were applicated once weekly with 1 µg TPA. Group E serves as a positive control, without being exposed but treated with 2 µg TPA twice weekly.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: 6 h/day, 5 days/week for 21 weeks

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Exposure duration 6 h/day, 5 days/week for 21 weeks
Exposure setup
Exposure source
  • specified in reference article
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2 mT - - - -

Reference articles

  • Stuchly MA et al. (1991): Cancer promotion in a mouse-skin model by a 60-Hz magnetic field: I. Experimental design and exposure system.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

Group A and B were used to determine whether the applied magnetic field could act like a classical tumor promoter. No papilloma development could be detected in these groups. Also no significant effect between these groups could be observed concerning NK cell activity.
Group C and D were used to determine if the applied magnetic field (together with the TPA application) could act as a tumor co-promoter. However, no statistically significant difference in tumor development could be found between these groups. The only effect demonstrated by this study was a significantly greater proportion of mice with enlarged spleens and increased number of mononucleated spleen cells in group D compared with the sham exposed group C.

Study character:

Study funded by

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