EMF-Portal | Proteomic response of Schizosaccharomyces pombe to static and oscillating extremely low-frequency electromagnetic fields

Proteomic response of Schizosaccharomyces pombe to static and oscillating extremely low-frequency electromagnetic fields med./bio.

By: Sinclair J, Weeks M, Butt A, Worthington JL, Akpan A, Jones N, Waterfield M, Allanand D, Timms JF

Published in: Proteomics 2006; 6 (17): 4755-4764

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Aim of study (acc. to author)

To study the effects of static and oscillating extremely low frequency electromagnetic fields on the proteomes of wild type yeast Schizosaccharomyces pombe and a deletion mutant which displays increased sensitivity to a variety of cellular stresses.

Endpoint

Exposure

Exposure	Parameters
Exposure 1: DC/static Exposure duration: continuous for 60 min	magnetic flux density: 1 mT unspecified
Exposure 2: 50 Hz Exposure duration: continuous for 60 min	magnetic flux density: 1 mT unspecified

Exposure 1

Main characteristics

Frequency	DC/static
Type	magnetic field
Exposure duration	continuous for 60 min

Exposure setup

Exposure source	solenoid
Setup	Four 100 ml conical flasks were placed in the gap provided by the coils where the MF was almost uniform. The currents in the coils were passed in opposite direction to get no MF for sham exposure.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	1 mT	unspecified	measured	-	-

Exposure 2

Main characteristics

Frequency	50 Hz
Type	magnetic field
Waveform	sinusoidal
Exposure duration	continuous for 60 min

Exposure setup

Exposure source	same setup as exposure 1

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	1 mT	unspecified	measured	-	-

Exposed system:

intact cell/cell culture
Schizosaccharomyces pombe (wild type and Sty1p deletion mutant)

Methods Endpoint/measurement parameters/methodology

Investigated system:

isolated bio./chem. substance
cell lysates

Time of investigation:

after exposure

Main outcome of study (acc. to author)

Whilst the data identified a number of protein isoforms that displayed significant differential expression across experimental conditions, there was no correlation between their patterns of expression and the extremely low frequency electromagnetic field exposure. The authors conclude that there are no significant effects of either static or oscillating electromagnetic fields on the yeast proteome (the authors hypothesise that the proteins identified must be sensitive to subtle changes in culture and/or handling conditions).

Study character:

medical/biological study
experimental study
full/main study
blind study

Study funded by

EMF Biological Research Trust (EMFBRT), UK

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Lin KW et al. (2016): Exposure of ELF-EMF and RF-EMF Increase the Rate of Glucose Transport and TCA Cycle in Budding Yeast

Huwiler SG et al. (2012): Genome-wide transcription analysis of Escherichia coli in response to extremely low-frequency magnetic fields

Chen G et al. (2012): Using model organism Saccharomyces cerevisiae to evaluate the effects of ELF-MF and RF-EMF exposure on global gene expression

Proteomic response of Schizosaccharomyces pombe to static and oscillating extremely low-frequency electromagnetic fields med./bio.

Aim of study (acc. to author)

Endpoint

Exposure

Exposure 1

Main characteristics

Exposure setup

Parameters

Exposure 2

Main characteristics

Exposure setup

Parameters

Methods Endpoint/measurement parameters/methodology

Main outcome of study (acc. to author)

Study funded by

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