The effects of exposure of Irpex lacteus, a white-rot fungus, to a 50 Hz magnetic field on gene expression profile and amino acid content should be investigated.
The authors previously found that an extremely low frequency magnetic field increased the biomass of Irpex lacteus and altered the surface strcuture (no reference specified). The present study should provide insights into the underlying mechanism of action.
Irpex lacteus cultures were allocated to two groups: an exposure group and a control group. The groups were studied at different times after exposure (recovery time): 0 hours, 3 hours and 6 hours. At each time point, 3 replicate flasks of each group were examined.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
50 Hz
Exposure duration:
3 hours per day for 4 days
|
|
| Frequency | 50 Hz |
|---|---|
| Type | |
| Exposure duration | 3 hours per day for 4 days |
| Exposure source |
|
|---|---|
| Chamber | flasks on a shaking table with coils in a small room (ca. 5 m2) with 28°C ambient temperature |
| Setup | system was composed of a shaking table with copper wire coils, the power supply and voltage regulator; six cylindrical coils with the same numbers of turns were fixed on the shaking table; one flask was placed in each coil during exposure (= simultaneous exposure of six flasks); temperature in flasks was 28 ± 1°C |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 3.5 mT | - | measured | - | ± 0.2 mT |
In exposed samples, 3,268, 1,377 and 941 genes were identified after recovery times of 0 hours, 3 hours, and 6 hours, respectively, which were significantly differently expressed compared to the control group. From these, 30 upregulated and 14 downregulated genes overlapped at all time points and the results were validated by quantitative RT-PCR. The identified genes related to several pathways but a considerably large number was involved in the metabolism pathways. This result was confirmed by a significantly increased amino acid content in exposed samples compared to the control samples (significance stated by authors, not comprehensible).
The authors conclude that exposure of Irpex lacteus to a 50 Hz magnetic field increases the amino acid content and that gene expression analysis can explain these changes.
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