Study type: Medical/biological study (experimental study)

Exposure of ELF-EMF and RF-EMF Increase the Rate of Glucose Transport and TCA Cycle in Budding Yeast. med./bio.

Published in: Front Microbiol 2016; 7: 1378

Aim of study (acc. to author)

The effects of exposure of yeast cells to a 50 Hz or 2 GHz electro-magnetic field on the global gene expression should be investigated.

Background/further details

Cells were divided into 3 groups: 1) exposure to the 50 Hz field, 2) exposure to the 2 GHz field, 3) control group.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 96 hours
Exposure 2: 2 GHz
Modulation type: CW
Exposure duration: continuous for 96 hours

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 96 hours
Exposure setup
Exposure source
Chamber plates placed between coils in incubator
Setup two combined solenoid systems were placed in incubator; each solenoid was 15 cm in length and 40 cm in diameter and was wrapped with 260 turns of single-strand copper wire; the distance between the closest ends of the two coils was 15 cm; temperature remained stable at 30°C
Additional info the incubator for the control group was placed in an area where the magnetic field was equal to the background level
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 6 mT - measured - -
electric field strength 205 V/m - measured - -

Exposure 2

Main characteristics
Frequency 2 GHz
Type
Waveform
Exposure duration continuous for 96 hours
Modulation
Modulation type CW
Exposure setup
Exposure source
Chamber plates
Setup the antenna of the exposure system was placed 24 cm above the sample area; the temperature was stable at 30°C
Parameters
Measurand Value Type Method Mass Remarks
SAR 0.12 W/kg mean calculated cf. remarks for a single cell
electric field strength 20 V/m - measured - at position of cells

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

A total of 8 genes was found in group 1 (50 Hz exposure) which were significantly upregulated while 1 gene was significantly downregulated compared to the control group. In group 2 (2 GHz exposure), a total of 7 genes was significantly upregulated while 3 genes were significantly downregulated compared to the control group.
Further analysis revealed that both exposures significantly upregulated the expression of genes involved in glucose transportation and the citric acid cycle, but not the glycolysis pathway.
The authors conclude that exposure of yeast cells to a 50 Hz or 2 GHz electro-magnetic field might increase the gene expression rate of genes involved in cell metabolism.

Study character:

Study funded by

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