A pilot study including 224 animals was conducted to select propper doses of TPA and to identify environmental effects on biomarkers.
The primary study includes a total of 192 animals, divided into 8 groups (24 animals each). Groups were treated either with none TPA or with doses of 0.85 nmol, 1.7 nmol, or 3.4 nmol TPA respectively. All mice were initiated by a single application of 10 nmol DMBA and TPA was administrated twice a week. The biomakers (i.e. epidermal thickness, enzyme activity and so on) were evaluated after 1, 2 and 5 weeks of TPA- induced promotion.
Exposure duration: 6 h/day, 5 days/week, up to 5 weeks
|Sham exposure||A sham exposure was conducted.|
|magnetic flux density||2 mT||-||-||-||-|