32 rats were divided in one experimental and one control group. Spleen cells of exposed rats were partly stimulated with IL-2 (after exposure; IL-2 can active the JAK-STAT signal transduction pathway).
The JAK/STAT signal transduction pathway plays an important role in regulating gene expression and cellular activation.
The data showed that the phosphor-STAT3 expression was remarkably up-regulated in spleen cells stimulated by IL-2 and the electromagnetic field, whereas the phosphor-STAT3 expression was slightly up-regulated in these spleen cells stimulated only by IL-2 (in the control group). The findings indicate that the electromagnetic fields of high-voltage lines accelerate the process of phosphorylation of STAT3 activated by IL-2, thus it activate also the JAK-STAT signal transduction pathway.