The effects of pulsed electric fields exposure on the mural and human cells were investigated in four different ion milieus and by the use of propidium iodide. The uptake of the fluorescent dye propidium iodide into the cell was measured as it is a sensitive marker of cell membrane integrity. The occurence of this dye in the cell indicates loss of membrane integrity. In this way mechanisms of changed membrane permeabilization under exposure to nanosecond pulsed electric fields should be further characterized.
|Pulse width||60 ns|
|electric field strength||12 kV/cm||-||calculated||-||-|
|cf. remarks||-||-||calculated||-||U = 540 - 580 V|
After short nanosecond pulsed electric fields exposure an increase in membrane conductance was observed in the cells and necrotic changes appeared after the exposure to long nanosecond pulsed electric fields. Both parameters were cell type-dependent. They were much weaker in human cervical carcinoma cells than in murine pituitary cells.
No propidium iodide uptake was observed by either cell line. Lanthanum and gadolinium ions significantly inhibited the nanosecond pulsed electric fields-induced membrane conductance increase, and protected the exposed cells from developing necrosis.