The detailed summary of this article is not available in your language or incomplete. Would you like to see a complete translation of the summary? Then please contact us →
This study was undertaken to analyze the effects of nanosecond pulsed electric fields exposure on cell membrane permeabilization in mural and human cells in vitro.
The effects of pulsed electric fields exposure on the mural and human cells were investigated in four different ion milieus and by the use of propidium iodide. The uptake of the fluorescent dye propidium iodide into the cell was measured as it is a sensitive marker of cell membrane integrity. The occurence of this dye in the cell indicates loss of membrane integrity. In this way mechanisms of changed membrane permeabilization under exposure to nanosecond pulsed electric fields should be further characterized.
Modulation type: pulsed
ばく露時間: up to 200 pulses
|ばく露時間||up to 200 pulses|
|Pulse width||60 ns|
|Distance between exposed object and exposure source||5 µm|
|ばく露装置の詳細||cells placed in the middle of the electrode gap|
|Sham exposure||A sham exposure was conducted.|
|参照||-||-||計算値||-||U = 540 - 580 V|
After short nanosecond pulsed electric fields exposure an increase in membrane conductance was observed in the cells and necrotic changes appeared after the exposure to long nanosecond pulsed electric fields. Both parameters were cell type-dependent. They were much weaker in human cervical carcinoma cells than in murine pituitary cells.
No propidium iodide uptake was observed by either cell line. Lanthanum and gadolinium ions significantly inhibited the nanosecond pulsed electric fields-induced membrane conductance increase, and protected the exposed cells from developing necrosis.