Study type: Medical/biological study (experimental study)

SIMS Study of the Calciumdeprivation step related to epidermal meristem production induced in flax by cold shock or radiation from a GSM telephone. med./bio.

Published in: Journal of Trace and Microprobe Techniques 2002; 20 (4): 611-623
Journal partially peer-reviewed/unclear

Aim of study (acc. to author)

This study was designed to investigate whether microwaves emitted by GSM mobile phones possibly exert influence on plants.

Background/further details

A sensitive experimental system was used: week-old seedlings of flax are subjected to a temporary calcium deprivation. This results in the production of epidermal meristems (meristems are small groups of actively dividing cells) in the hypocotyls (part of the stem between the root and the first leaves), typically 10-20 meristems after a month, provided the seedlings have previously undergone an exposure to different stresses or stimuli (such as drought or cold shock). In control experiments designed to minimize these stresses, the mean number of meristems produced per plant is 0 or 1 after subjecting seedling to calcium deprivation.
Cold shock treted plants were the positive control. Further investigations were performed on ion distribution of the epidermis and cortical tissue of the mid part of flax hypocotyl during calcium deprivation.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 0.9 GHz
Modulation type: pulsed
Exposure duration: continuous for 2 h
-

General information

seedlings were treated in three groups: i) EMF exposure from GSM phone on day 6 ii) cold schock for 1 min on day 6 iii) control = no treatment

Exposure 1

Main characteristics
Frequency 0.9 GHz
Type
Exposure duration continuous for 2 h
Modulation
Modulation type pulsed
Exposure setup
Exposure source
Distance between exposed object and exposure source 17 cm
Setup low intensity sound was delivered to the phone's microphone using a miniature headphone to maintain emission from the phone; this assembly was wrapped in absorbent laboratory paper
Parameters

No parameters are specified for this exposure.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Flax seedlings responded to the combination of either a 2 hours GSM telephone irradiation or a 1 minute cold shock plus a 2-day calcium deprivation in the same way as they respond to other combinations such as touch, wind or drought plus calcium deprivation.
Significant changes in ion distribution were observed for calcium, magnesium, sodium and potassium. Flax seedlings tended to maintain the ratio of divalent to monovalent cations constant during calclium deprivation

Study character:

Study funded by

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