A sensitive experimental system was used: week-old seedlings of flax are subjected to a temporary calcium deprivation. This results in the production of epidermal meristems (meristems are small groups of actively dividing cells) in the hypocotyls (part of the stem between the root and the first leaves), typically 10-20 meristems after a month, provided the seedlings have previously undergone an exposure to different stresses or stimuli (such as drought or cold shock). In control experiments designed to minimize these stresses, the mean number of meristems produced per plant is 0 or 1 after subjecting seedling to calcium deprivation.
Cold shock treted plants were the positive control. Further investigations were performed on ion distribution of the epidermis and cortical tissue of the mid part of flax hypocotyl during calcium deprivation.
Modulation type: pulsed
Exposure duration: continuous for 2 h
|Exposure duration||continuous for 2 h|
No parameters are specified for this exposure.
Flax seedlings responded to the combination of either a 2 hours GSM telephone irradiation or a 1 minute cold shock plus a 2-day calcium deprivation in the same way as they respond to other combinations such as touch, wind or drought plus calcium deprivation.
Significant changes in ion distribution were observed for calcium, magnesium, sodium and potassium. Flax seedlings tended to maintain the ratio of divalent to monovalent cations constant during calclium deprivation