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In order to determine if there was a dose response relationship between electromagnetic fields applied and the amount of transcript accumulated, plants were also exposed at others doses and durations. Leaf tissue was harvested at 15, 30, and 60 minutes after exposure (in some experiments also immediately and after 5 minutes).
The MSRC (mode stirred reverberation chamber) consisted of a large room (200 m³) with a double-layered steel wall, acting as a Faraday cage, an antenna, and movable blades to stir the EMF. Tomato plants were grown inside plywood culture chambers. Shielded chambers were enclosed in a polymer mesh covered with aluminium foil to protect the plants from exposure [Vian et al., 2006].
When the plants were 3 weeks old, the whole culture chamber was placed in the MSRC (24 h prior to irradiation) in a determined working volume where the field was homogeneous and isotropic.
The fourth terminal leaf (exposed and shielded) was harvested at different times after exposure (0, 5, 15, 30, and 60 min).
Roux D et al.
Article Addendum: A possible role for extra-cellular ATP in plant responses to high frequency, low amplitude electromagnetic field
Roux D et al.
High frequency (900 MHz) low amplitude (5 V m-1) electromagnetic field: a genuine environmental stimulus that affects transcription, translation, calcium and energy charge in tomato.