Study type: Medical/biological study (experimental study)

Exposure to 50 Hz electromagnetic fields induces the phosphorylation and activity of stress-activated protein kinase in cultured cells. med./bio.

Published in: Electro Magnetobiol 2001; 20 (3): 415-423

Aim of study (acc. to author)

To study the effects of a 50 Hz electromagnetic field on the cell signal transduction process.

Background/further details

The phosphorylation of stress-activated protein kinase was measured. Cells were exposed in the two intensitiy groups. Every intensity group was divided into eight duration subgroups (3, 5, 10, 15, 30 min and 1, 12, 24 h), and a sham exposure group. All subgroup cells were harvested at the same time (4 days after beginning of exposure).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 3 min, 5 min, 10 min, 15 min, 30 min, 1 h, 12 h, 24 h

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 3 min, 5 min, 10 min, 15 min, 30 min, 1 h, 12 h, 24 h
Exposure setup
Exposure source
Setup 3 square Helmholtz coils (36 cm x 8 cm) with 168, 60, 168 (upper, middle, lower coil) turns of 1.16 mm copper wire, connected in series, set up inside an iron-shielded box inside a CO2 incubator; culture bottles placed in the central area between the coils
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.4 mT - measured - -
magnetic flux density 0.8 mT - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Both 0.4 mT and 0.8 mT could induce the phosphorylation of stress-activated protein kinase. The phosphorylation of stress-activated protein kinase presented a time-dependent course. The phosphorylated stress-activated protein kinase enhanced its enzymatic activity.
All the data indicated that 50 Hz electromagnetic field could activate stress-activated protein kinase in a time- and intensity-dependent manner. The biological effects caused by 50 Hz electromagnetic fields may be related to the stress-activated protein kinase signal transduction pathway.

Study character:

Study funded by

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