Study type: Medical/biological study (experimental study)

The role of protein kinase SAPK/JNK in cell responses to low-intensity nonionizing radiation. med./bio.

Published in: Biophysics 2009; 54 (2): 179-181

Aim of study (acc. to author)

To study the effect of low-intensity laser light (He-Ne, 0.2 mW/cm², 632.8 nm, exposure time 1 min) and of superhigh frequency electromagnetic fields (8.15-18 GHz, 1 µW/cm², exposure time 1 h) on phospho-SAPK/JNK production in mouse spleen lymphocytes.

Background/further details

To block intracellular Hsp90 proteins, geldanamycin (1 µg/ml) was used.



Exposure Parameters
Exposure 1: 8.15–18 GHz
Exposure duration: continuous for 1 h

General information

Cells were treated in three groups: i) exposure to low-intensity laser light ii) exposure to centimeter waves iii) control In a second experiment the same groups were used but cells were pre-incubated with geldanamycin.

Exposure 1

Main characteristics
Frequency 8.15–18 GHz
Exposure duration continuous for 1 h
Exposure setup
Exposure source
  • sweep frequency generator with a sweep time of 1 s
Measurand Value Type Method Mass Remarks
power density 1 µW/cm² mean - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data showed a significant increase of phospho-SAPK/JNK production in lymphocytes after exposure to laser light or microwaves in both cell groups (with/without geldanamycin). Exposure to superhigh frequency electromagnetic fields produced a more significant effect (greater increase) compared to the low-intensity laser light exposure.
The authors concluded that activation of the SAPK/JNK signal pathway plays one of the central roles in cellular stress response to low-power non-ionizing radiation.

Study character:

Study funded by

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