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Medical/biological study (experimental study)

Electric field exposure and evidence of stress in mice.

Published in: Environ Res 1994; 65 (1): 149-160

Aim of study (acc. to author)

To examine the effect of stress induced by an electric field in six generations of mice.
Background/further details: Six consecutive generations consisted of ten control pairs of adult mice and ten exposed pairs of adult mice in each generation. The examination of the adrenal glands was performed on young mice (35 days old, n=10), adults (6 months old, n=20) and old mice (18 months, n=10). Blood samples were taken from adult male mice (daytime and nighttime) as well as male and female old mice (only daytime) (n=12 in each group).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 22 hr/day from conception untill death (35 days - 18 months)
Exposure 1
Main characteristics
Frequency 50 Hz
Type
Exposure duration 22 hr/day from conception untill death (35 days - 18 months)
Exposure setup
Exposure source
  • two parallel capacitor plates placed horizontally and 600 mm apart
Setup Perspex animal cages placed on the lower capacitor plate 180 mm inside the edge
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 10 kV/m - - - -
Exposed system:
  • animal
  • mouse/BALB/c
  • whole body

Methods Endpoint/measurement parameters/methodology

Investigated material:
Investigated organ system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

The mean lipid volume in the zona glomerulosa of the adrenal glands was significantly higher in the exposed adult male group than in the control group. The daytime corticosterone level of the exposed adult male mice was also significantly higher than that of the controls. The lipid profiles and corticosterone values in all other subgroups did not differ significantly.
Study character:

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