Study type: Medical/biological study (experimental study)

Chilling resistance of Phaseolus vulgaris and Brassica oleracea under a high-intensity electric field. med./bio.

Published in: Z Naturforsch C 2010; 65 (5-6): 380-386

Aim of study (acc. to author)

To study the effects of an electric field, low temperature, and their combinations on tissue vitality and some physiological variables regarding antioxidant responses of cold-sensitive bean and cold-tolerant kale leaves.

Background/further details

15 day old seedlings were exposed to an electric field prior to cold treatment (5/2°C day/night). Plants were harvested on 3rd and 6th day after electric field application.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 10 min. or 40 min.

General information

Leaves were treated in six groups: i) control ii) 10 min. exposure iii) 40 min. exposure iv) cold treatment v) 10 min. exposure + cold treatment vi) 40 min. exposure + cold treatment

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration 10 min. or 40 min.
Exposure setup
Exposure source
  • two parallel aluminum plates with a diameter of 50 cm and a distance of 20 cm
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 100 kV/m - calculated - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

In both plants, cold application alone caused statistically significant increments in total protein levels and the antioxidant enzyme activities. However, tissue vitality and hydrogen peroxide levels did not change in the cold-tolerant kale, while tissue vitality decreased and hydrogen peroxide levels increased in cold-sensitive bean.
Electric field application alone did not cause any significant changes in the both plant species. On the other hand, 40 min electric field exposure increased the deteriorative effect of cold treatment in both plant species, while 10 min electric field augmented the chilling resistance by increasing the tissue vitality and antioxidant enzyme activities resulting in decreased hydrogen peroxide levels.
The authors conclude that electric field exposure (100 kV/m) for a very short time (10 min) before chilling treatment augments the chilling resistance by elevating the enzymatic antioxidant activation and tissue vitality, thus resulting in less hydrogen peroxide formation especially in cold-sensitive species.

Study character:

Study funded by

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