Diabetic rats were created by the injection of 45 mg/kg streptozotocin, a toxin which selectively destroys the beta cells in the pancreas. Healthy rats received a (sham) injection with citrate buffer.
60 rats were divided into 4 groups (n=15 each): 1) exposure of healthy rats to the magnetic field, 2) exposure of diabetic rats to the magnetic field, 3) healthy rats control group, 4) diabetic rats control group.
After exposure, all rats were killed and the thoracic aorta was isolated. 3-4 mm long aorta rings were prepared and placed in a bath with test solution including either a vasoconstrictor (10-7 M phenylephrine) or vasodilator (10-6 M acetylcholine or 10-6 M sodium nitroprusside, respectively).
|magnetic flux density||5 mT||-||measured||-||-|
(Remark EMF-Portal: the statistical significance of the results was not stated clearly in the text and tables and could therefore not be regarded in the following.)
The weight of healthy rats (groups 1 and 3) increased over time, whereas the weight of diabetic rats (groups 2 and 4) decreased over time.
In healthy and diabetic rats exposed to the magnetic field (groups 1 and 2) in comparison to their corresponding control groups (groups 3 and 4), the vasoconstriction force decreased after addition of phenylephrine while the vasodilation increased after addition of acetylcholine, but not after addition of sodium nitroprusside.
The authors conclude that chronic exposure of healthy and diabetic rats to a 50 Hz magnetic field might have an effect on vasoconstriction and vasodilation of isolated thoracic aorta rings.