Study type: Medical/biological study (experimental study)

Effect of Extremely Low Frequency Electromagnetic Fields (EMF) on Phospholipase Activity in the Cultured Cells. med./bio.

Published in: Korean J Physiol Pharmacol 2010; 14 (6): 427-433

Aim of study (acc. to author)

To study the effect of extremely low frequency electromagnetic fields (60 Hz, 0.1 or 1 mT) on cellular signal pathway, by observing the changes of phospholipase enzyme activities in two different cell lines.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: continuous for 4 or 16 h

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Exposure duration continuous for 4 or 16 h
Exposure setup
Exposure source
Setup four square coils; cage with three floors arranged vertically; exposure system shielded with ferrite material
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.1 mT - measured - -
magnetic flux density 1 mT - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The basal and melittin-induced arachidonic acid release (phospholipase A2 enzyme activity) was not affected by the electromagnetic field exposure in both cell lines. There was no change of cytosolic phospholipase A2 and secreted phospholipase enzyme activity due to exposure. Also both phospholipase C and phospholipase D enzyme activities did not show any change in the two exposed cell lines.
The data indicate that the exposure to electromagnetic fields which is 2.4 fold higher than the limit value of occupational exposure does not induce phospholipase-associated signal pathway in RAW 264.7 cells and RBL 2H3 cells.

Study character:

Study funded by

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