To study the effect of extremely low frequency electromagnetic fields (60 Hz, 0.1 or 1 mT) on cellular signal pathway, by observing the changes of phospholipase enzyme activities in two different cell lines.
Exposure duration: continuous for 4 or 16 h
|Exposure duration||continuous for 4 or 16 h|
|magnetic flux density||0.1 mT||-||measured||-||-|
|magnetic flux density||1 mT||-||measured||-||-|
The basal and melittin-induced arachidonic acid release (phospholipase A2 enzyme activity) was not affected by the electromagnetic field exposure in both cell lines. There was no change of cytosolic phospholipase A2 and secreted phospholipase enzyme activity due to exposure. Also both phospholipase C and phospholipase D enzyme activities did not show any change in the two exposed cell lines.
The data indicate that the exposure to electromagnetic fields which is 2.4 fold higher than the limit value of occupational exposure does not induce phospholipase-associated signal pathway in RAW 264.7 cells and RBL 2H3 cells.