The enzyme activity was examined under different conditions: 1.) no treatments, 2.) magnetic field exposure, 3.) variations of pH value, 4.) variations of pH value + magnetic field exposure, 5.) variations of temperature and 6.) variations of temperature + magnetic field exposure.
|Exposure duration||continuous for 20 min|
|Setup||pair of Helmholtz coils mounted on a wooden frame 7 cm apart with a PVC test tube stand to hold the samples in the center; each coil with an inner radius of 7 cm, an outer radius of 10 cm and 500 turns of copper wire with a diameter of 0.25 mm|
|Sham exposure||A sham exposure was conducted.|
In exposed samples (group 2), the enzyme activity was 50% higher when compared to the unexposed samples (group 1). Magnetic field exposure alone (group 2) led to a significantly increased Michaelis-Menten constant and a significantly increased maximum reaction velocity compared to the samples without treatment (group 1). No significant differences were observed for the optimal pH value (group 4 compared to group 3) and for the optimal temperature (group 6 compared to group 5) between exposed and unexposed samples.
The authors conclude that exposure to extremely low frequency magnetic fields increased the enzyme activity of the alpha amylase.