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To investigate the effect of exposure to extremely low frequency magnetic fields on the enzyme activity of the alpha amylase.
The enzyme activity was examined under different conditions: 1.) no treatments, 2.) magnetic field exposure, 3.) variations of pH value, 4.) variations of pH value + magnetic field exposure, 5.) variations of temperature and 6.) variations of temperature + magnetic field exposure.
| ばく露 | パラメータ |
|---|---|
|
ばく露1:
50 Hz
ばく露時間:
continuous for 20 min
|
|
| 周波数 | 50 Hz |
|---|---|
| タイプ |
|
| ばく露時間 | continuous for 20 min |
| ばく露の発生源／構造 | |
|---|---|
| ばく露装置の詳細 | pair of Helmholtz coils mounted on a wooden frame 7 cm apart with a PVC test tube stand to hold the samples in the center; each coil with an inner radius of 7 cm, an outer radius of 10 cm and 500 turns of copper wire with a diameter of 0.25 mm |
| Sham exposure | A sham exposure was conducted. |
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 磁束密度 | 0.53 mT | effective value | 測定値 | - | - |
In exposed samples (group 2), the enzyme activity was 50% higher when compared to the unexposed samples (group 1). Magnetic field exposure alone (group 2) led to a significantly increased Michaelis-Menten constant and a significantly increased maximum reaction velocity compared to the samples without treatment (group 1). No significant differences were observed for the optimal pH value (group 4 compared to group 3) and for the optimal temperature (group 6 compared to group 5) between exposed and unexposed samples.
The authors conclude that exposure to extremely low frequency magnetic fields increased the enzyme activity of the alpha amylase.
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