研究のタイプ: 医学/生物学の研究 (experimental study)

[アルファアミラーゼ活性に対する50Hz電磁界の影響] med./bio.

Effect of 50 Hz electromagnetic fields on Alpha amylase activity.

掲載誌: Romanian J Biophys 2008; 18 (3): 255-263

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研究目的(著者による)

To investigate the effect of exposure to extremely low frequency magnetic fields on the enzyme activity of the alpha amylase.

詳細情報

The enzyme activity was examined under different conditions: 1.) no treatments, 2.) magnetic field exposure, 3.) variations of pH value, 4.) variations of pH value + magnetic field exposure, 5.) variations of temperature and 6.) variations of temperature + magnetic field exposure.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 20 min

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 20 min
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 pair of Helmholtz coils mounted on a wooden frame 7 cm apart with a PVC test tube stand to hold the samples in the center; each coil with an inner radius of 7 cm, an outer radius of 10 cm and 500 turns of copper wire with a diameter of 0.25 mm
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.53 mT effective value 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

In exposed samples (group 2), the enzyme activity was 50% higher when compared to the unexposed samples (group 1). Magnetic field exposure alone (group 2) led to a significantly increased Michaelis-Menten constant and a significantly increased maximum reaction velocity compared to the samples without treatment (group 1). No significant differences were observed for the optimal pH value (group 4 compared to group 3) and for the optimal temperature (group 6 compared to group 5) between exposed and unexposed samples.
The authors conclude that exposure to extremely low frequency magnetic fields increased the enzyme activity of the alpha amylase.

研究の種別:

研究助成

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