Study type: Medical/biological study (experimental study)

Modulation of the catalytic activity of free and immobilized peroxidase by extremely low frequency electromagnetic fields: dependence on frequency. med./bio.

Published in: Bioelectromagnetics 2005; 26 (2): 145-152

Aim of study (acc. to author)

To investigate the effects of extremely low frequency electromagnetic fields on the catalytic activity of the enzyme horseradish peroxidase.

Background/further details

Horseradish peroxidase is a membrane bound enzyme in vivo and by this means to consider as insoluble. The catalytic activity of soluble as well as artificial insoluble peroxidase was investigated in this study in vitro. Immobilization was achieved either by entrapment on a gelatin membrane or by covalent attachment on a nylon membrane.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50–400 Hz
Exposure duration: 1, 2, 3 and 4 h

Exposure 1

Main characteristics
Frequency 50–400 Hz
Type
Waveform
Exposure duration 1, 2, 3 and 4 h
Exposure setup
Exposure source
Setup A Plexiglas shelf held the samples parallel to the coils and was positioned in the middle of the structure between the two coils.
Additional info Two coils were framed on a Plexiglas structure parallel to each other at distance of 10 cm. The temperature during the experiment was kept at 25°C.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT effective value measured - -

Reference articles

  • Portaccio M et al. (2003): In vitro studies of the influence of ELF electromagnetic fields on the activity of soluble and insoluble peroxidase.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure

Main outcome of study (acc. to author)

For soluble peroxidase a positive as well as a negative percentage change of the catalytic activity was found caused by electromagnetic field exposure. A positive maximum was noticed at 130 Hz. Depending on the character of immobilization a maximum positive effect was found at 150 Hz (entrapment) and at 170 Hz (covalent attachment), respectively. No negative interaction could be found for insoluble peroxidase.

Study character:

Study funded by

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