Study type: Medical/biological study (experimental study)

Can exposure to manganese and extremely low frequency magnetic fields affect some important elements in the rat teeth? med./bio.

By: Ince B, Akdag Z, Bahsi E, Erdogan S, Celik S, Akkus Z, Dalli M, Sahbaz C, Akdogan M, Kara R, Yavuz Y, Gullu V, Gunay A, Guven K
Published in: Eur Rev Med Pharmacol Sci 2012; 16 (6): 763-769
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Aim of study (acc. to author)

To examine the potential correlation between exposure to 50 Hz magnetic fields and/or the application of manganese and the elements calcium, zinc, magnesium, and phosphorus in rat teeth.

Background/further details

The mineral content in teeth is associated with dental caries.
Rats were divided in 8 groups (n=8 per group): 1.) 60 mg manganese per kg body weight + exposure, 2.) 15 mg manganese per kg body weight + exposure, 3.) 3.75 mg manganese per kg body weight + exposure, 4.) exposure, 5.) 60 mg manganese per kg body weight, 6.) 15 mg manganese per kg body weight, 7.) 3.75 mg manganese per kg body weight and 8.) cage control group.
Manganese application and exposure were repeated daily for 45 days.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous 4 h/day during 45 days

General information

rats were treated in the following eight groups: i) exposure to 60 mg/kg Mn + exposure to EMF ii) exposure to 15 mg/kg Mn + exposure to EMF iii) exposure to 3.75 mg/kg Mn + exposure to EMF iv) exposure to EMF only v) exposure to 60 mg/kg Mn vi) exposure to 15 mg/kg Mn vii) exposure to 3.75 mg/kg Mn viii) control group

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous 4 h/day during 45 days
Exposure setup
Exposure source
Setup two pairs of Helmholtz coils positioned opposite one another on the vertical axis inside a flexiglass cage; animal cage placed inside a Faraday cage
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Rats receiving a manganese application and/or were exposed to a magnetic field had significantly increased levels of zinc and magnesium and signifcantly decreased levels of calcium and phosphorus in comparison to the cage control group.
The authors conclude that application of manganese and/or exposure to extremely low frequency magnetic fields could influence the mineral content of rat teeth, which could lead to an increased risk of dental caries.

Study character:

Study funded by

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