Study type: Medical/biological study (experimental study)

The effects of a 1.8 GHz continuous electromagnetic fields on mucociliary transport of human nasal mucosa. med./bio.

Published in: Laryngoscope 2013; 123 (2): 315-320

Aim of study (acc. to author)

To investigate the effects of a 1.8 GHz electromagnetic field (CW) on the human nasal muco-ciliary transport and to determine the pathophysiology of ciliary beat frequency.

Background/further details

Muco-ciliary transport is a major respiratory tract host defense mechanism that works against inhaled microbes and noxious materials. Cilia movement is an essential point for this fundamental defense mechanism. The ciliary beat frequency is a quantitative measurement of ciliary beats and the most important factor responsible for regulating muco-ciliary transport.
Nasal mucosa (mucosa + cells) was obtained from 18 patients (10 males, 8 females). One sample was obtained from each of three patients and each measurement was performed three times. The cultured nasal mucosa was used for measurement of the ciliary beat frequency with and without protein kinase C (PKC) inhibitor (agents that activate PKC have been associated with decreasing ciliary beat frequency). Normal nasal epithelial cells were used to examine the cytotoxicity of exposure to an 1.8 GHz electromagnetic field.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 1.8 GHz
Modulation type: CW
Exposure duration: continuous for 3 days

Exposure 1

Main characteristics
Frequency 1.8 GHz
Type
Exposure duration continuous for 3 days
Modulation
Modulation type CW
Exposure setup
Exposure source
Setup sample plates placed inside an incubator directly above the dipole antenna in the H-field maxima
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
power 1 W - - - -
SAR 1 W/kg - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

In nasal mucosa cells of the exposed group, the ciliary beat frequency began to decrease compared to the control samples from the beginning of the exposure with a maximum difference after three days. A significant increase in the enzyme activity of protein kinase C was observed in exposed samples in comparison to control samples. An incubation of the exposed sample with the protein kinase C inhibitor Calphostin C abolished the exposure induced decrease of the ciliary beat frequency. The addition of the protein kinase C inhibitor Gö-6976 did not affect the ciliary beat frequency neither in the exposed nor in the control group. However, the addition of the protein kinase C inhibitor GF 109203X prevented any exposure induced decrease of the ciliary beat frequency.
In nasal epithelial cells, no significant difference was observed in the cell viability between the exposed samples and the control samples.
The authors summarize that exposure to a 1.8 GHz electromagnetic field may inhibit the ciliary beat frequency via an "novel protein kinase C" dependent mechanism, but seems not to be cytotoxic in nasal epithelial cells.

Study character:

Study funded by

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