Newly eclosed insects were separated into 12 identical groups of ten males and ten females and exposed (at 3 different intensities) or sham exposed continuously during the first 5 days of their adult lives. For the first 48 hours male and female flies were put in different glass vials. After the 48 h, when both males and females of each group were sexually mature, they were put together (10 pairs) in another glass vial and they were allowed to mate and lay eggs for the next 72 h. After these 5 days, the flies were removed from the vials. The female flies were dissected and ovaries studied for DNA damage. The vials with the developing embryos were incubated in the culture room for 6 additional days without exposure.
glass tubes with flys were put in the centre of the air cores of the coils, suspended by nylon strings
Setup
two nearly identical cylindrical coils were used; both coils had a length of 0.25 m, a radius of 7.5 x 10-2 m and a diameter of insulated wire of 2 x 10-3 m; number of turns in each coil = 330; in the first coil, the turns were parallel and in the same direction, thus generating a magnetic field; in the second coil, half of the turns were antiparallel (parallel but in opposite direction), so that the magnetic field (and the magneticallyinducedelectric field) in a region of about 12 cm width around the centre of the coil was zero (used for sham exposure)
after it was checked by preliminary experiments that the sequence (mutual position) of the two coils did not affect the outcome of the experiments, their positions were always the same during all experiments, at a certain place of the laboratory with the minimum stray 50-Hz fields and in at least 2 m distance between each other so that the fields of the "exposurecoil" did not affect the "sham exposurecoil"
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