Study type: Medical/biological study (experimental study)

P-selectin expression in a colon tumor model exposed by sinusoidal electromagnetic fields. med./bio.

Published in: Biomed Rep 2013; 1 (3): 389-392

Aim of study (acc. to author)

To investigate the protein expression level of P-selectin in a colon tumor model exposed to a 50 Hz-magnetic field.

Background/further details

P-selectin is a protein which functions as a cell adhesion molecule on the surfaces of endothelial cells and has a functional role in metastasis of tumor.
Rats were divided into four groups: 1.) control group (n=5), 2.) weekly rectally carcinogen administration (2 mg N-Methyl-N-Nitrosurea dissolved in 0.2 ml isotonic solution, for 10 weeks) (n=7), 3.) magnetic field exposure (n=9) and 4.) weekly rectally carcinogen administration (2 mg N-Methyl-N- Nitrosurea dissolved in 0.2 ml isotonic solution, for 10 weeks) + magnetic field exposure (n=7). The control group and the magnetic field exposure group were treated with saline solution without carcinogen in the same way.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 6 hours/day for 8 month

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 6 hours/day for 8 month
Exposure setup
Exposure source
Chamber rats were housed in a polycarbonate cage
Setup 12 copper solenoid coils (560 turns, resepctively) were serially connected, cores of the coils were filled with soft iron rods and tightened to increase the magnetic flux intensity, coils were vertically placed and the cages were held 1.2 cm above the coils to diminish vibration, 1 cm thick wooden plates were placed between the coils and cages to avoid heating, coils were connected to the 220 V 50 Hz sinusoidal city electric systems
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 5 mT - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

No significant differences in the protein expression level of P-selectin were observed between the groups as well as no differences regarding food consumption.
The authors conclude that additional studies are required to verify the results.

Study character:

Study funded by

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