The effects on DNA, with focus on single-strand breaks, in different types of cells in brain, kidney and liver of mice after a chronic whole body exposure to 50 Hz magnetic fields should be investigated.
86 mice were separated into the following three groups: 1) 26 animals as sham exposure, 2) 30 animals exposed to a magnetic field with 0.1 mT und 3) 30 animals exposed to a magnetic field with 1.0 mT. After exposure, 10 animals per group were chosen randomly (the remaining animals were not investigated in this study) and DNA damage was investigated.
|Chamber||30 mice were kept in metal free cages (14 cm height, 16 cm width, 22 cm depth) in groups of five per cage and exposed simultaneously for each magnetic flux density tested|
|Setup||a pair of identical circular Helmholtz coils with a diameter of 69 cm and separated by a distance of 34.5 cm|
|Sham exposure||A sham exposure was conducted.|
|magnetic flux density||0.1 mT||-||measured||-||-|
Neither the exposure to the magnetic field with 0.1 mT nor with 1.0 mT resulted in any increase of unrepaired nuclear DNA single-strand breaks in any cell types investigated compared to sham exposure. However, the exposure with 1.0 mT led to significantly reduced unscheduled DNA synthesis in one type of brain and kidney cells, as well as to significantly reduced mitochondrial DNA synthesis in both types of brain cells.
The authors conclude that the results indicate no increase of single-strand breaks as an effect of a chronic whole body exposure of mice to 50 Hz magnetic fields up to 1.0 mT.