研究のタイプ: 医学／生物学の研究 (experimental study)

[成獣マウスの脳、腎臓、肝臓の明らかに異なるタイプの細胞において磁束密度0.1mTまたは1.0mTの50Hz磁界の8週間連続ばく露後に未修復の核DNA一本鎖切断が残存する証拠はない] med./bio.

No evidence of persisting unrepaired nuclear DNA single strand breaks in distinct types of cells in the brain, kidney, and liver of adult mice after continuous eight-week 50 Hz magnetic field exposure with flux density of 0.1 mT or 1.0 mT

著者: Korr H, Angstman NB, Born TB, Bosse K, Brauns B, Demmler M, Fueller K, Kantor O, Kever BM, Rahimyar N, Salimi S, Silny J, Schmitz C
掲載誌: PLoS One 2014; 9 (10): e109774
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この研究の著者らは、磁束密度1.5mTの50Hz磁界へ8週間連続ばく露した後の成獣マウスの脳と腎臓の多数のタイプの細胞を調べ、脈絡叢上皮細胞でのみ未修復の核DNA一本鎖切断（nDNA SSB）が増加したことを報告した（Acta Neuropathologica 2004; 107: 257-264）。今回は、磁束密度0.1mTまたは1.0mTで同様の結果が得られるかを､前回と全く同じ実験方法で検証した。その結果、脳、腎臓、肝臓からの明らかに異なるタイプの細胞において、未修復のnDNA SSB残存の増加はなかった､と報告している。

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研究目的（著者による）

The effects on DNA, with focus on single-strand breaks, in different types of cells in brain, kidney and liver of mice after a chronic whole body exposure to 50 Hz magnetic fields should be investigated.

詳細情報

86 mice were separated into the following three groups: 1) 26 animals as sham exposure, 2) 30 animals exposed to a magnetic field with 0.1 mT und 3) 30 animals exposed to a magnetic field with 1.0 mT. After exposure, 10 animals per group were chosen randomly (the remaining animals were not investigated in this study) and DNA damage was investigated.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 8 weeks
ばく露2: 50 Hz
ばく露時間: continuous for 8 weeks

General information

experimental set-up differes from reference set-up due to a different amount of test animals and a different magnetic flux density used

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 8 weeks
ばく露装置
ばく露の発生源／構造
チャンバの詳細 30 mice were kept in metal free cages (14 cm height, 16 cm width, 22 cm depth) in groups of five per cage and exposed simultaneously for each magnetic flux density tested
ばく露装置の詳細 a pair of identical circular Helmholtz coils with a diameter of 69 cm and separated by a distance of 34.5 cm
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 0.1 mT - 測定値 - -
Additional parameter details

the magnetic fields generated in the laboratory by the 50 Hz power supply were lower than 0.2 µT. In the control cages, the maximal flux density of 50 Hz magnetic fields was 0.15 µT. The earth's DC magnetic field was below 50 µT in all areas of the investigations.

ばく露2

主たる特性
周波数 50 Hz
ばく露時間 continuous for 8 weeks
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 1 mT - 測定値 - -

Reference articles

  • Schmitz C et al. (2004): [50Hz磁界ばく露は成獣マウスの脳および腎臓の明らかにタイプの異なる細胞でのDNA修復およびミトコンドリアDNA合成に影響する]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Neither the exposure to the magnetic field with 0.1 mT nor with 1.0 mT resulted in any increase of unrepaired nuclear DNA single-strand breaks in any cell types investigated compared to sham exposure. However, the exposure with 1.0 mT led to significantly reduced unscheduled DNA synthesis in one type of brain and kidney cells, as well as to significantly reduced mitochondrial DNA synthesis in both types of brain cells.
The authors conclude that the results indicate no increase of single-strand breaks as an effect of a chronic whole body exposure of mice to 50 Hz magnetic fields up to 1.0 mT.

研究の種別:

研究助成

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